Activation of the alternative pathway of complement by grain. I. C3PA conversion and quantification of complement consumption by rye.
Olenchock-SA; Mull-JC; Major-PC; Gladish-ME; Peach-MJ III; Pearson-DJ; Elliott-JA; Mentnech-MS
Clin Allergy 1978 Mar; 8(2):125-133
The possible relationships between grain dust and activation of the alternative pathway of complement were addressed. Ground rye and airborne rye dust were added to normal human serum after particle size measurements were made. Reaction mixtures were incubated and tested for conversion of proactivator of the third component of complement (C3PA) to the active form (C3A), and for hemolytic complement activity (CH). Both rye samples, which were of similar size, with 70 to 75 percent of the particles being less than 10 microns, activated C3PA conversion to C3A with a dose response effect over concentration of 0.1 to 20 milligrams (mg). Airborne rye (5mg) consumed 100 percent of CH, while 5mg ground rye consumed 47 percent. The authors conclude that rye dust has the potential to activate complement in a dose dependent manner in both sensitized and nonsensitized individuals.
NIOSH-Author; Respiratory-system-disorders; Respiratory-irritants; Lung-irritants; Toxic-materials; Grain-elevator-workers; Airborne-dusts; Airborne-particles; Particulates