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Toxicity of ultraviolet-irradiated halothane in mice.
O'Neal FO; Menzel DB; Wells CP; Sabransky M; Karis JH
Toxicology 1982 Feb; 23(2-3):223-234
The toxicity of halothane (151677) was investigated. Male CD1-mice were exposed for 90 minutes to an atmosphere containing 1.3 percent halothane irradiated by ultraviolet (UV) light or to nonirradiated halothane. After 24 hours, half the mice received 50 milligrams per kilogram of sodium-pentobarbital (57330) and loss of righting reflex and sleeping time were recorded. Liver microsomes were prepared. Protein and enzyme assays were conducted. Additional mice were exposed to 1 percent irradiated or nonirradiated halothane and injected 24 hours later with carbon-14 (C-14) labeled sodium- pentobarbital. Urine and feces were collected and metabolites determined. Sleeping time for mice receiving irradiated halothane increased from 14.3 to 72.5 minutes. Mixed function oxidase activity increased. Only a small amount of C-14 was excreted after 24 hours in mice exposed to UV treated halothane. Serum glutamic oxalacetic transaminase and serum glutamic pyruvic transaminase were 233 and 377 percent of control values, respectively. Hepatic cytochrome P-450 and microsomal aminopyrine demethylase activities decreased in mice exposed to UV irradiated halothane. The authors conclude that debromination and formation of 2,2,2-trifluroacetyl- chloride (354325) are responsible for the increased toxicity of UV irradiated halothane.
NIOSH-Publication; NIOSH-Grant; Toxicology; Comparative-toxicology; Lung-disorders; Analytical-methods; Analytical-chemistry; Hepatotoxicity; Laboratory-animals; Enzyme-activity
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