Microbial flora and fauna of respirable grain dust from grain elevators. Combined mycological and entomological evaluation of grain dust components.
Whidden MP; Smalley EB; Caldwell RW; Phillips JK; Mai S; Burkholder WE
Morgantown, WV: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Institute for Occupational Safety and Health, Contract 210-77-0150, 1980 Dec; :1-120
Fungus spores, bacteria, mite and insect parts, and pesticide residues in grain dust were evaluated as possible causal agents in chronic, nonspecific lung disease. Respirable dust samples were obtained from 250 workers in eight different grain elevators. Grain dust was analyzed for microbial viable counts, direct counts, grain dust challenge studies, and scanning electron microscopy. Pure culture toxicity determinations on small animals and the entomological analysis of grain dust and bulk grain were addressed. Bacteria were the most prevalent microorganisms in airborne grain dust, and the predominant fungi in respirable grain dust were yeasts. The major fungi present produce mycotoxins, to which the authors attribute some of the adverse health effects experienced by the workers. The authors conclude that examination of grain dust by scanning electron microscopy is a most revealing technique for examination of airborne grain dust.
NIOSH-Contract; Contract-210-77-0150; Microorganisms; Vegetable-dusts; Dust-analysis; Dust-sampling; Mycology
Final Contract Report
National Institute for Occupational Safety and Health
University of Wisconsin