Tier II Mutagenic Screening of 13 NIOSH Priority Compounds: Individual Compound Report Cyclohexanone.
NIOSH 1980 Nov:202 pages
The mutagenicity of cyclohexanone (108941) was studied in a tier II mutagenic test screening program using CD-rats and B6C3F1 hybrid mice. The assays used included the unscheduled DNA synthesis (UDS) assay in human diploid fibroblasts with exposures of 3 hours (h) duration and concentrations up to 9.48 milligrams per milliliter of culture medium; the dominant lethal test in male rats following atmospheric exposure to 50 parts per million (ppm) or 400 ppm of cyclohexane for 7h per day for 5 days; sperm abnormality tests in male mice using the same exposure conditions as in the dominant lethal test; the cytogenetic test in male and female rat bone marrow cells using exposure conditions similar to the dominant lethal test or after single exposure for 7h followed by sampling after 6, 24, and 48h; and the sex linked recessieve lethal (SLRL) test in Drosophila-melanogaster after inhalation exposure to 50ppm for 7h or 400ppm for 40 minutes. There was no increase in UDS in cells treated with cyclohexanone. There were no effects attributable to cyclohexanone in the dominant lethal test and abnormal sperm frequency was not affected. There was no significant increase in chromosomal aberrations in the rat bone marrow cells and SLRL frequency was not increased in Drosophila-melanogaster. The authors conclude that cyclohexanone did not cause any observable genetic damage in these bioassays.
NIOSH-Contract; Contract-210-78-0026; Solvents; Laboratory-animals; Screening-methods; Mutagenesis; Genetic-disorders; Biochemical-tests;
NTIS Accession No.
NIOSH, Cincinnati, Ohio, Inveresk Research International Limited, Musselburgh, Scotland, 202 pages, 17 references