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Development of sampling and analytical methods for carcinogens, January 1 - September 30, 1976.

Morales R; Rappaport SM; Weeks RW Jr.; Campbell EE
Los Alamos Scientific Laboratory of the University of California, Los Alamos, New Mexico 1977 Dec; :27
The development and testing of air sampling and analysis methods to detect benzidine (92875), benzidinium-sulfate, 3,3'- dichlorobenzidine (91941), 3,3'-dichlorobenzidine-dihydrochloride (612839), alpha-naphthylamine (134327), and beta-naphthylamine (91598), and the evaluation of commercial respirator cannisters for use with 4,4'-methylenebis(2-chloroaniline) (101144) and the naphthylamines are described. Test atmospheres were obtained by passing the benzidines through a nebulizer and the naphthylamines through a vapor diffuser. Benzidines were collected on a sampler with a filter and 50 milligrams (mg) silica gel. The naphthylamine sampler had a filter followed by 100 and 50mg sorbent sections. Benzidines were eluted with a solution of triethylamine and methyl- alcohol. Naphthylamines were desorbed with a mixture of acetic-acid and 2-propanol. Benzidines and naphthylamines were analyzed by high performance liquid chromatography (HPLC) and gas chromatography (GC), respectively. Respirator cannisters were tested in atmospheres containing 100 to 160 and 100 to 300 micrograms per cubic meter 4,4'-methylenebis(2-chloroaniline) and naphthylamines, respectively. Temperature did not effect sampler capacity. Increasing relative humidity decreased alpha-naphthylamine adsorption and interaction of temperature and humidity significantly altered beta-naphtylamine adsorption. The range of the analytical method for benzidine 3,3'-dichlorobenzidine, and naphthylamine, was 3 to 130 and 3 to 140, and 4 to 70 micrograms per cubic meter, respectively for 50 liter (l) air samples. The precision of the sampling and analysis method showed significant differences in variances for the naphthylamines but not for the benzidines. The respirator cannisters successfully retained the carcinogens tested. The authors conclude that the procedures tested should be adequate for commercial needs with possible modifications. The tested cannisters are capable of protecting workers from benzidine and 3,3'- dichlorobenzidine.
NIOSH-Contract; Contract-IA-74-35; Air-monitoring; Monitors; Air-sampling-equipment; Analytical-instruments; Sampling-methods; Analytical-processes; Air-sampling; Sampling-equipment
92-87-5; 91-94-1; 612-83-9; 134-32-7; 91-59-8; 101-14-4
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Los Alamos Scientific Laboratory of the University of California, Los Alamos, New Mexico
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