A method for the sampling and analysis of wood dust was tested for NIOSH validation. A 37 millimeter type AE Gelman glass filter was preconditioned by sampling clean air, heating to 105 degrees C, and equilibrating in a controlled environment. The filter was weighed and the sample collected. The loaded filter was dried at 105 degrees C and weighed again. The protocol for establishing NIOSH validation was to analyze sample filters spiked with appropriate amounts of wood dust and samples collected from dynamically generated test atmospheres, test the collection efficiency of the glass fiber filters, determine the storage stability of spiked and generated filters, and assess the precision and accuracy of the method. The aerosol generation and sampling system was presented. The total error for sampling and analysis was less than 25 percent at the NIOSH recommended standard 95 percent of the time. The authors conclude that this method satisfies NIOSH criteria for validation.