Detection of environmental depigmenting substances.
Gellin GA; Maibach HI; Misiaszek MH; Ring M
Contact Dermatitis 1979 Aug; 5(4):201-213
The depigmenting capacity of some phenols, catechols, and organic antioxidants was studied. Test materials were applied to epilated and unepilated skin sites of black guinea-pigs for 1 to 6 months, and to unepilated dorsal surfaces of black mice for 2 to 4 months. If significant irritation developed, treatment was discontinued until irritation subsided. Biopsies were performed on treated and control sites at the conclusion of treatment. Clear cut depigmentation was seen with monomethyl-ether of hydroquinone (123319), and tertiary-butyl-catechol (27213781) in both guinea-pigs and mice, however the damage was less pronounced in mice. Full depigmentation of the back was seen for p-tertiary-amyl-phenol and of the nipple for monobenzyl-ether of hydroquinone (123319) in guinea-pigs. Application of isopropyl-catechol (28801345), hydroquinone (123319), p-tertiary-butyl-phenol (98544), phenol (108952), and catechol (120809) resulted in moderate depigmentation. False negative responses with known depigmenting chemicals were observed. The authors propose a screening procedure for detecting the depigmenting capacity of medicinal and industrial chemicals, involving the use of five guinea pigs per test agent, chemical application to the back of the animal, use of solvent control groups, and a minimum test period of 60 days.
NIOSH-Publication; NIOSH-Grant; Laboratory-animals; Pigmentation-disorders; Skin-exposure; Skin-disorders; Comparative-toxicology; Medical-screening; Screening-methods
Dermatology University of California Department of Dermatology San Francisco, Calif 94122
123-31-9; 27213-78-1; 123-31-9; 28801-34-5; 123-31-9; 98-54-4; 108-95-2; 120-80-9
University of California San Francisco, San Francisco, California