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Formation of free radical intermediates during nitrous oxide metabolism by human intestinal contents.
Bosterling B; Trudell JR; Hong K; Cohen EN
Biochem Pharmacol 1980; 29(21):3037-3038
The effects of nitrous-oxide (10024972) on the formation of free radical intermediates during metabolism by human intestinal contents were studied. Nitrous-oxide was incubated with 200 milligrams of human intestinal contents in the presence of either 5,5-dimethyl-1- pyrroline-N-oxide or phenyl-N-tert-butylnitrone (PBN) as a spin trap. Free radicals were detected by electron paramagnetic resonance (EPR). Complex EPR spectra suggesting several trapped radicals resulted from a 5 hour incubation in which anaerobiosis was produced by beta-D-glucose, glucose-oxidase, and catalase. Incubation with nitrous-oxide produced concentrations of trapped free radicals 3 to 20 times greater than those produced by the argon control. Nitrous-oxide had no effect on hydroxyl radical PBN adducts once these were formed. The authors conclude that the hydroxyl radical adducts observed after 20 hours result from hydrolysis of less stable nitrous-oxide radical adducts rather than from nitrous-oxide stimulation of endogenous production of hydroxyl radicals. They suggest that some of the toxic effects of nitrous- oxide may be due to formation of nitrous-oxide radicals in the body.
NIOSH-Publication; NIOSH-Grant; Nitrogen-oxides; Gastrointestinal-system; Biochemical-analysis; Biochemistry; Toxicology; Metabolites
Anesthesia Stanford University 300 Pasteur Drive Stanford, Calif 94305
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Stanford University, Stanford, California
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