Development of a photodynamic bioassay to establish an index for the carcinogenic hazard to man of polynuclear aromatic hydrocarbons and their heterocyclic analogs and application of time-resolved phosphorimetry to the qualitative determination of PNA's and their heterocyclic analogs.
NIOSH 1972 Jun; :1-111
A photodynamic bioassay was used to establish an index for the carcinogenic hazard of polynuclear-aromatic-hydrocarbons (PNAs) and their heterocyclic analogs. Time resolved phosphorimetry techniques for use in determining the PNAs in mixtures also were developed. The bioassay involved use of true PNA solutions of known concentrations and monochromatic light, with Artemia salina as the test organism. Relative photodynamic activity (RPA) was determined for 18 known carcinogens and 23 noncarcinogens after 2 or 22 hour dark incubation periods. Benz(c)acridine (225514) was used as the standard and was assigned an activity of 1.0. RPA correlated very well with carcinogenicity for both incubation periods. Most compounds with an RPA equal to or greater than 1.0 were known carcinogens. Bioassay of nine coal tar pitch volatile air samples gave RPAs of 0.115 to 1.14, but conclusions were not drawn about the hazard of these mixtures because their carcinogenic potency was not known. The authors conclude that the RPA of PNAs is a good indicator of their carcinogenicity and that time resolved phosphorimetry can be used to calculate the carcinogenicity of mixtures of compounds.
NIOSH-Contract; Contract-099-72-0077; Bioassays; Aromatic-hydrocarbons; Analytical-methods; Carcinogenesis; Qualitative-analysis; Biochemical-analysis; Physical-properties; Polynuclear-aromatic-hydrocarbons; Coal-tar-pitch
Department of Chemistry, University of Cincinnati, Cincinnati, OH 45221
Final Contract Report
NTIS Accession No.
National Institute for Occupational Safety and Health
University of Cincinnati, Cincinnati, Ohio