Cellular response to coal in vitro (CWP).
Christian-RT; Elia-VJ; Cody-TE; Menefee-MG; Bell-M
Department of Environmental Health, Anatomy and Pathology, University of Cincinnati, 1982 Jan; :1-8
Cellular responses to coal dust were studied. Coal samples from mines in Pennsylvania and Utah were placed in contact with cultures of continuous line mouse cells (L-929) and continuous line normal and primary human cells. The toxicity of coal to mammalian cells and the effect of coal particle size were determined. Simple leachates of coal were toxic to cells, and coal samples from a mine in which the workers had a very high incidence of coal workers pneumoconiosis (CWP) inhibited cell growth much more than coal from other mines. Cellular toxicity paralleled the prevalence of CWP in the two mines. Nickel (7440020) was present in Pennsylvania coal leachate in a much higher concentration than in the Utah coal leachate, but did not account for all of the toxic differences between the samples. Coal toxicity was independent of particle size. Rapidly growing L- cells were the most sensitive and stationary phase primary human cells were the least sensitive to the toxic chemicals in coal. The authors conclude that cell culture techniques are very useful for studying environmental toxicants.
NIOSH-Grant; Pulmonary-system-disorders; Cellular-reactions; Coal-dust; Cell-cultures; Toxicology; Mammalian-cells; Cytology; Cell-damage; Dust-exposure
Environmental Health University of Cincinnati Eden & Bethesda Avenues Cincinnati, Ohio 45219
Final Grant Report
NTIS Accession No.
Department of Environmental Health, Anatomy and Pathology, University of Cincinnati
University of Cincinnati, Cincinnati, Ohio