Leukocyte recruitment through airway walls by condensed vegetable tannins and quercetin.
Kilburn-KH; Lynn-WL; Tres-LL; McKenzie-WN
Lab Invest 1973 Jan; 28(1):55-59
The effects of extracts from cotton mill refuse, cotton dust and quercetin (117395) on leukocyte recruitment through airway walls were studied in hamsters. Animals were exposed to aerosols containing 1 or 0.1 percent solution of extracts for 4 or 6 hours and killed 1, 2, 4, 6, 12 or 24 hours after starting exposure. Each hamster was anesthetized and a tube was inserted into the trachea while osmium-tetroxide in fluorocarbon flowed into the lungs. Breathing ceased in 5 minutes, and tissues were prepared. One hour after beginning of exposure to a 1 percent solution, airway cells were normal; by 2 hours polymorphonuclear cells (PMNs) were lining the basal lamina. After 4 hours, many hamsters had leucocytes between many airway epithelial cells, beneath the basal lamina and on the luminal surfaces. The ratio of PMNs associated with airways to airway epithelial cells was 0.22 hours after the beginning of exposure, to 1 percent solution 0.36 after 4 hours and 0.12 after 6 hours. When the condensed complex polyphenols were refined, leucocyte recruitment activity was enhanced so that exposure to 0.1 percent solution for 4 hours produced 1.19 PMN per airway compared to a ratio of 0.81 after 4 hours exposure to a 1 percent solution of the crude extract. Hamsters exposed to lint free dust had a few PMN cells at 6 hours but all layers were infiltrated by leucocytes at 12 hours. With quercetin, the cellular recruitment was similar to that in response to cardroom dust, but cells appeared beneath the basal lamina at 6 hours.
NIOSH-Publication; NIOSH-Grant; Laboratory-animals; Cotton-dusts; Biological-transport; Transport-mechanisms; Dust-exposure; Cellular-reactions; Blood-cells; Cell-migration; Flavones
Medicine Duke University ED Ctr Durham, N C 27706
Duke University, Durham, North Carolina