The effects of chlordane, DDT, and 3-methylcholanthrene upon the metabolism and toxicity of diethyl-4-nitrophenyl phosphorothionate (parathion).
Toxicol Appl Pharmacol 1971 Apr; 18(4):977-987
The effect of chlordane (57749), DDT (50293), and 3- methylcholanthrene (56495) (MCA) on the enzyme systems of parathion (56382) metabolism and toxicity was studied in Holtzman-rats and CF- 1-mice. DDT and chlordane were given at a dose of 100 milligrams per kilogram (mg/kg) per day for 3 days, while MCA was administered intraperitoneally in a dose equivalent to 1 percent of the body weight. Animals were killed after 24 hours, and liver homogenates were prepared. Metabolites of (diethyl-C-14)parathion were isolated and identified by thin layer chromatography. Diethyl-hydrogen- phosphorothionate (DHP) production from parathion was stimulated 210 percent in DDT treated rats and the production of paraoxon was stimulated 206 percent. Chlordane stimulated DHP and paraoxon 146 and 166 percent, respectively. MCA resulted in a 83 percent stimulation in DHP and a 182 percent stimulation in paraoxon. The addition of DDT to mouse liver fraction resulted in an enhancement of the pathway responsible for DHP production. A pattern of equal stimulation of both pathways in chlordane-treated rats was observed with mice. MCA administration produced a significant 49 percent depression of DHP production. The author concludes that parathion is metabolized by two separate mixed-function oxidase systems.
NIOSH-Publication; NIOSH-Grant; Pesticides-and-agricultural-chemicals; Laboratory-animals; Toxicology; Comparative-toxicology; Metabolism; Enzyme-activity
Pharmacology and Therapeutics Univ of Florida Coll of Med Dept of Pharma & Therapeutics Gainesville, Fla 32601
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Toxicology and Applied Pharmacology
University of Florida Gainesville, Gainesville, Florida