Heterogeneity of intrafollicular somatic cells and ovulated cumulus masses as evidenced by delta5-3beta-HSDH activity.
Histochemistry 1980 Jan; 68:39-48
The localization of delta-5-3-beta-hydroxysteroid-dehydrogenase (HSDH) was studied in CD-1-mouse ovaries. The tissue was incubated with steroid substrate, dehydrated, embedded in glycol-methacrylate and formazan deposition was determined by light microscopy. HSDH substrates studied included etiocholanolone (ETCH) and dehydroisoandrosterone (DHAS). Pregnant mare serum gonadotropin (PMSG) was administered to some mice 10 hours before sacrifice. The HSDH reaction observed near the basement membrane was more pronounced than in cells nearer the antrum. Cumulus masses were observed in PMSG treated mice that resembled the cells surrounding ovulated oocytes. Ovulated oocyte-cumulus cell masses were well preserved histologically and many but not all gave strong evidence of HSDH when ETCH was used as the substrate, while DHAS elicited a much weaker reaction. The authors conclude that this method allows the histochemical determination of HSDH and should help clarify the role played by the different cellular components in oocyte maturation, atresia and follicular growth and selection.
NIOSH-Publication; NIOSH-Grant; Histology; Cellular-reactions; Laboratory-animals; Sample-preparation; Analytical-methods;
Anatomy Louisiana State University 1542 Tulane Avenue New Orleans, LA 70112
Louisiana State University, School of Medicine, Department of Anatomy, New Orleans, Louisiana