Properties and drug sensitivity of adenosine triphosphatases from Schistosoma mansoni.
Nechay-BR; Hillman-GR; Dotson-MJ
J Parasitol 1980 Aug; 66(4):596-600
The effects of ions and antischistosomal drugs on adenosine- triphosphatase (ATPase) activity in Schistosoma-mansoni were investigated. Infected ICR-mice were sacrificed when their infections were about 6 weeks old. Worm pairs were recovered by dissection and placed in a cell culture medium. Some worms were homogenized immediately while others were incubated for 4 hours. The incubation mixture contained diluted tissue homogenates, sodium- chloride (7647145), potassium-chloride (7447407), calcium-chloride (10043524), and magnesium-chloride (7786303) in various concentrations. Praziquantel (55268741) and hycanthone-mesylate were dissolved in the medium for incubation of the worms, or in water for in-vitro ATP studies. ATPase was strongly activated by magnesium and calcium. A maximum increase occurred at 150 millimolar sodium. When added together, potassium did not increase the enzyme activity beyond that shown by calcium alone. Sodium and potassium by themselves or in combination did not stimulate the enzyme unless calcium or magnesium were present. The maximum calcium or magnesium enhancement of ATPase activity occurred at pH 8.0. Ouabain produced a maximum inhibition of 17 percent of schistosomal ATPase under optimal ionic conditions for sodium, potassium and magnesium induced ATPase activity. Niridazole, hycanthone, oxaminiquine, praziquantel and antimony-potassium- tartrate had no direct effect.
NIOSH-Publication; NIOSH-Grant; Metabolism; Enzyme-activity; Biochemistry; Laboratory-animals; Synergism; Dose-response; Pharmaceuticals; Parasitic-diseases
Pharmacology and Toxicology University of Texas Pharm & Toxicology Department Galveston, Tex 77550
7647-14-5; 7447-40-7; 10043-52-4; 7786-30-3; 55268-74-1
Journal of Parasitology
University of Texas Med BR Galveston, Galveston, Texas