Response of early chick embryos to busulfan.
Teratology 1980 Jan; 21(1):1-8
The mutagenic and toxic effects of busulfan (55981) were studied in white-Leghorn-chick embryos. Busulfan was administered to embryos in doses of either 0.1, 0.5 or 1.0 milligram (mg) at 24, 48, 72, or 96 hours of incubation. Groups of embryos exposed to either 0.5 or 1.0mg of busulfan early in development had a high rate of mortality. Of the 86 embryos in this class, only 12 survived to 5 days. Abnormalities in limb bud development in the form of pointed distal ends were common in survivors. No defects were observed in embryos exposed to busulfan at 96 hours. At a dose of 0.1mg given at 24 or 48 hours, 10 of the 36 survivors developed cephalic hematomas and 7 had defects in limb bud development. Embryos treated with busulfan at 24 and 48 hours of incubation had a much higher mortality rate than those exposed at 72 or 96 hours. The authors conclude that busulfan in doses of 0.5 and 1.0mg is very lethal to chick embryos when administered prior to incubation at 24 or 48 hours.
NIOSH-Publication; NIOSH-Grant; Biochemistry; Physiology; Dose-response; Mutagens; Laboratory-animals; Age-factors; Reproductive-effects; Embryopathology
William J. Swartz, Department of Anatomy, Louisiana State University, 1542 Tulane Avenue, New Orleans, LA 70112
Louisiana State University, School of Medicine, Department of Anatomy, New Orleans, Louisiana