Effect of excess and deficient copper intake on rat liver microsomal enzyme activity.
Biochem Pharmacol 1973; 22(12):1463-1476
The effects of copper loading and deficiency on basal levels of hepatic microsomal enzyme activity were studied in Holtzman-rats. In the copper loading study, rats were given water supplemented with 50, 150, or 450 parts per million (ppm) of cupric-sulfate (7758987) for 15 and 30 days. In the copper deficiency studies, rats were administered a commercial diet containing only 1 to 2 milligrams of copper per kilogram of diet. Phenobarbital-sodium (57307) solutions were administered daily to subgroups of copper deficient animals for 3 days prior to sacrifice. In copper loaded rats, liver glucose 6- phosphatase and benzpyrene-hydroxylase activities were not significantly changed, while liver aniline hydroxylase activity was reduced in the 450ppm group to approximately 20 percent of the control value at both 15 and 30 days. In copper deficient rats, a significant decrease in liver aniline hydroxylase activity was observed at 21 days and continued throughout the 42 day test period. Hexobarbitol sleeping time was prolonged at both 21 and 42 days. The reduction of the metabolism of aniline in-vitro and the metabolism of hexobarbitol both in-vitro and in-vivo was reversed by restoration of copper to the diet.
NIOSH-Publication; NIOSH-Grant; Grants-other; Metabolism; Enzyme-activity; Biochemistry; Biosynthesis; Biotransformation; Metallic-poisons; Body-burden; Heavy-metals
Physiology Harvard University 665 Huntington Ave Boston, Mass 02115
Other Occupational Concerns; Grants-other
Harvard University, Boston, Massachusetts