The NIOSH analytical method S307 for determining warfarin (81812) in air is described. Samples are collected on a polytetrafluoroethylene filter, extracted with methanol, and analyzed by high performance liquid chromatography with an ultraviolet detector at 280 nanometers. The working range of the method is 0.054 to 0.244 milligrams per cubic meter, the coefficient of variance is 0.056, and the detection limit is about 0.5 micrograms per milliliter for a 20 microliter injection. Analytical apparatus, reagents and procedures are described, and calculations are provided for determining sample concentrations. Data on particle size distribution, collection efficiency, and recovery and stability rates is included. The method was unsatisfactory because the accuracy could not be adequately determined.
NIOSH, Unpublished, Cincinnati, Ohio, 24 pages, 5 references