State of the Art: Chemically Induced Mammalian Cell Mutagenesis and Transformation.
NIOSH 1980 Oct:135-177
In-vitro mammalian cell systems for detecting chemically induced mutagenicity and transformation are discussed. The following assays are reviewed: mammalian target cell systems; normal diploid rodent systems; epithelial cell systems; and human cell systems. The effect of metabolic activation is considered. Methods for selecting mutant cells in a treated population, such as mass selection, lethal growth and thymineless death are evaluated. Experimental results with various chemicals are tabulated based on test cell system, transformation criteria, percent of transformation, and major findings. Established and experimental in-vitro test systems are listed. The author notes that some of these systems can provide information on the relationship between mutagenicity and carcinogenicity.
In-vitro-study; Mammalian-cells; Biotransformation; Bioassays; Biochemistry; Analytical-methods; Genetics; Cancer;
Proceedings of a Workshop on Methodology for Assessing Reproductive Hazards in the Workplace, April 19-22, 1978, P.F. Infante and M.S. Legator, Eds. Division of Surveillance, Hazard Evaluations and Field Studies, NIOSH, U.S. Department of Health and Human Services