Complement, polymorphonuclear leukocytes and platelets in acute experimental respiratory reactions to Aspergillus.
Olenchock-SA; Mentnech-MS; Mull-JC; Gladish-ME; Green-FHY; Manor-PC
Comp Immunol Microbiol Infect Dis 1979 Jun; 2(1):113-114
The roles of neutrophilic polymorphonuclear leukocytes (PMN) and platelets in acute experimental respiratory reactions to Aspergillus spores were studied. Serum from male rabbits was assayed for precipitins against Aspergillus (A.) terreus by counterimmunoelectrophoresis. Partial pressure of arterial oxygen and carbon dioxide were measured before and after aerosol challenge with A. terreus. Assays for the hemolytic activity of complement were performed on plasma from the identical blood samples used for blood gas analysis. Nitrogen mustard (51752) (HN2) was injected intravenously at a concentration known to deplete circulatory PMN in rabbits and the injected animals were subsequently challenged with A. terreus aerosol. Normal rabbits selectively depleted of PNM by NH2 showed a decrease in platelets after challenge. A decrease was not seen in the similarly challenged untreated group or in the unchallenged HN2-treated animals. The authors conclude that platelets appear capable of initiating a similar, albeit delayed, reaction, which emphasizes the flexibility of pulmonary defense mechanisms against inhaled particles.
Blood-chemicals; Metabolic-processes; Laboratory-animals; Polymorphonuclear-leukocytes; Toxic-substances; Analytical-methods; Sensitivity-testing; Fungal-spores;
Author Keywords: Complement; alternative pathway; polymorphonuclear leukocytes; platelets; rabbits; nitrogen mustard; arterial oxygen tension; PaO2; Aspergillus; lung
Comparative Immunology, Microbiology and Infectious Diseases