NIOSHTIC-2 Publications Search
Trace analysis of potentially carcinogenic metabolites of an azo dye and pigment in hamster and human urine as determined by two chromatographic procedures.
Nony CR; Bowman MC
J Chromatogr Sci 1980 Feb; 18(2):64-74
Electron capture/gas chromatography (EC/GC) and high pressure liquid chromatography (HPLC) were used to detect the metabolites of Direct Black 38 (1937377) and Pigment Yellow 12 (6358856) in hamster and human urine. Analytic procedures included: extraction of the free aromatic amines and neutral compounds; alkaline hydrolysis of the aqueous phase and extraction of any hydrolyzed conjugates as free amines; and analysis of the free amines and acetylated metabolites directly by high pressure liquid chromatography or electron capture gas chromatography after conversion of the amines to heptafluorobutyryl derivatives. Metabolites in hamster and human urine were detected at concentrations as low as 1 part per billion. The authors recommend this approach for monitoring exposed workers since it will also detect any other derivatizable aromatic amines.
NIOSH-Publication; NIOSH-Contract; Contract-224-78-0004; Chemical-analysis; Analytical-methods; Dyestuffs; Monitoring-system; Coloring-materials; Industrial-factory-workers; Metabolic-effects; Carcinogens; Work-environment; Urinalysis
Issue of Publication
Journal of Chromatographic Science
Page last reviewed: December 11, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division