Mitogenic response to Micropolyspora faeni cell walls.
Smith-SM; Snyder-IS; Buyrrell-R
J Allergy Clin Immunol 1980 Apr; 65(4):298-304
The mitogenic response of splenic lymphocytes from unsensitized guinea pigs to cell wall fractions prepared from Micropolyspora faeni (M-faeni) was investigated. Three M-faeni cell wall fractions were incubated with lymphocyte preparations for 48 hours. All of the fractions were mitogenic for the lymphocytes, and the response was dose dependent. The most active cell wall fractions were primarily B cell mitogens. The heat stable mitogenic reactivity was associated with polypeptide and polysaccharide. The mitogenic potential of the reactive material decreased after lysozyme digestion, indicating that part of the material was peptidoglycan. The authors suggest that mitogenic reactivity is host related.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders; Respiratory-system-disorders; Organic-dusts; Extrinsic-allergic-alveolitis; Farmers-lung; Cell-division; Nuclear-chemistry; Cellular-reactions; Dose-response; Biological-responses
Microbiology West Virginia University Med C Department of Microbiology Morgantown, W VA 26506
Journal of Allergy and Clinical Immunology
West Virginia University, Morgantown, West Virginia