Antibody-independent complement activation by cardroom cotton dust.
Kutz SA; Olenchock SA; Elliott JA; Pearson DJ; Major P
Environ Res 1979; 19(2):405-414
The potential of cardroom cotton dust to activate in vitro the complement cascade from nonsensitized human and guinea pig sera was investigated. Untreated or treated (chlorophyll free, reduced lipid content, lyophilized) cotton dust samples were incubated with serum aliquots. Both dusts consumed hemolytic complement and reduced the C3 complement, but the untreated dust was much more active than the treated dust. In all serum samples, the untreated dust consumed greater amounts of complement or complement components than the treated dust. The untreated dust had 58 times more endotoxin than the treated dust. The alternative pathway was identified as the source for complement activation. The authors suggest that the higher concentration of endotoxin in the untreated dust cannot account for the total complement consumed, even though endotoxin activation of the alternative pathway has been documented. Cotton dust inhalation in vivo may induce airway changes mediated by complement activation.
NIOSH-Author; Cotton-textiles; Textile-workers; Textile-mills; Sensitization; Laboratory-animals; Respiratory-system-disorders; Invitro-study; Blood-chemistry; Toxicity; Immunoreaction