Metal Induced Lipid Peroxidation in Cell Membranes: Final Report on Project VKC-R22-147.
Resnick-H; Berardinelli-SP; Danner-J; Judy-D
NIOSH 1979:12 pages
An in-vitro screening system for metal toxicity was evaluated. Toxic effects of cadmium (7440439) (Cd), mercury (7439976) (Hg), zinc (7440666) (Zn), and selenium (7782492) (Se) on lipid peroxidative metabolism were investigated in rabbit alveolar macrophage cultures. After a 20 to 44 hour incubation period in less than 0.05 millimoles of the chlorides of Cd(2+), Zn(2+) and Hg(2+), there was no increase of malonaldehyde (542789) or in the activity of glutathione peroxidase and glutathione reductase. There was no effect after the same incubation period with concentrations of up to 0.03 millimoles of selenomethionine (1464422), but with concentrations as low as 0.0125 millimoles of sodium-selenite (10102188) there was significant toxicity to the cells. Preincubation of macrophages with Zn(2+) protected the cells against the disruptive action of Cd(2+), but the protective action could not be explained by any observed increase or decrease in lipid peroxidation. Selenomethionine and sodium selenite did not provide any protective action against the cellular disruption induced by Hg(2+) salt. The authors conclude that assaying for lipid peroxidative metabolism in macrophages is not useful for metal toxicity screening; however, this system may be useful for toxicity screening with particulates or chemicals.
In-vitro-study; Cytological-analysis; Toxicity; Chemical-exposure; Measurement-methods; Immunity; Lipids; Metabolic-effects; Airborne-particulates; Laboratory-animals;
7440-43-9; 7439-97-6; 7440-66-6; 7782-49-2; 542-78-9; 1464-42-2; 10102-18-8;
Biochemistry Section, Laboratory Investigations Branch, DRDS, ALOSH, NIOSH, Morgantown, West Virginia, 12 pages, 32 references