Toxicity of Halogenated Compounds on Lipid Bilayers: Report on Project VKC-C23-169.
Resnick-H; Berardinelli-SP; Danner-J
NIOSH 1979:10 pages
The perturbation of model lipid bilayer vesicles by halogenated hydrocarbons was investigated to determine if cellular membrane damage could be evaluated and used as a screening technique for toxicity. A fluorescent probe of 1-anilino-8-naphthalene-sulfonate (82768) (ANS) was bound to synthetic phospholipid vesicles prepared from egg lecithin and dimyristoyl-phosphatidyl-choline (DML). The fluorescence intensity of the probe decreased in the presence of a homologous series of the chlorinated aromatics of monochlorophenols, dichlorophenols, and pentachlorophenols (87865). The decrease in fluorescence correlated with toxicity of the compounds. Oral administration of monochlorinated phenols in rats at a concentration of 1.0 millimolar resulted in a fluoresence intensity decrease of 29 to 49 percent and toxicity ratios (LD50) ranging from 0.57 to 0.67 grams per kilogram. The authors conclude that the spectrofluorometric approach of using phospholipid vesicles provides a useful system for estimating the toxicity of halogenated hydrocarbons.
Hazardous-substances; Toxic-effects; Acute-exposure; Screening-tests; Cytotoxicity; Cell-membrane; Cell-damage; Analytical-methods; Fluorescence-spectrometry; Research-instrumentation;
Biochemistry Section, Laboratory Investigations Branch, DRDS, ALOSH, NIOSH, Morgantown, West Virginia, 10 pages, 16 references