Analysis of total arsenic in urine and blood by high speed anodic stripping voltammetry.
Davis-PH; Berlandi-FJ; Dulude-GR; Griffin-RM; Matson-WR; Zink-EW
Am Ind Hyg Assoc J 1978 Jun; 39(6):480-490
A method for measuring parts per billion concentrations of total arsenic (7440382) (As) in urine and blood was formulated. Blood and urine samples were wet ashed with sulfuric-acid, nitric-acid, and perchloric-acid, then reductillated (reduced and distilled) to convert As(V) to As(III) and to separate the As(III) from other ions in the sample matrix. The resulting solution was then analyzed for its As content by anodic stripping voltammetry (ASV) at a gold film electrode. The accuracy of the technique was evaluated by analyzing several standards of known As content. The agreement between values obtained by the test method and accepted values for the standards was excellent. Recovery of arsenic from whole blood also was outstanding, and required only 4 milliliters for analysis. Percent recovery of As from urine was acceptable. The time required for reductillation of samples was 12 minutes, and 2 minutes were necessary for ASV analysis. By simultaneous reductillation of four samples, parts per billion arsenic determinations could be accomplished at the rate of approximately 12 per hour. The authors conclude that the reductillation/ASV procedure is as accurate, precise, and reliable at the nanogram level as the more universally accepted colorimetric techniques are at the microgram and milligram levels.
NIOSH-Publication; NIOSH-Contract; Contract-099-72-0107; Industrial-poisons; Measurement-instrumentation; Detectors; Analytical-methods; Urinalysis; Blood-chemical-analysis; Reaction-products; Chemical-reactions; Electrochemical-analysis; Electrochemical-equipment
American Industrial Hygiene Association Journal