Extracts of airborne grain dusts activate alternative and classical complement pathways.
Olenchock-SA; Mull-JC; Major-PC
Ann Allergy 1980 Jan; 44(1):23-28
The action of airborne grain dust extracts on the alternative and classical complement pathways, in humans was studied. Airborne grain dust extracts were characterized in terms of protein nitrogen, and gram negative bacterial endotoxic contamination. Extracts were added to 0.5 milliliters of pooled normal human serum, and the aliquots were tested for complement activities, C3 conversion in chelated NHS, and chemotaxis of polymorphonuclear leukocytes. Extracts consumed hemolytic complement in a dose dependent manner. No relationship was determined between complement activity and either protein or endotoxin concentrations. Differential serum chelation with grain extracts proved that the alternative pathways were activated, while hemolytic titers of the early complement components indicated that the classical pathway was involved. Complement was required for extract treated sera, which was chemotactic for PMN. The authors conclude that extracts of airborne grain dusts can activate alternative and classical complement pathways in-vitro, and can generate complement dependent directional migration of human PMN.
NIOSH-Author; Airborne-dusts; Bacterial-dusts; Analytical-methods; Hematology; In-vitro-studies; Cytology; Pulmonary-system-disorders
Annals of Allergy