Chemical characterization of uncoated asbestos fibers from the lungs of asbestos workers by electron microprobe analysis.
Langer-A; Rubin-I; Selikoff-I; Pooley-F
J Histochem Cytochem 1972; 20(9):735-740
Lung tissue specimens from anthophyllite (17068789), amosite (12172735), and crocidolite (12001284) asbestos workers were analyzed to determine whether uncoated amphibole asbestos fibers remain chemically intact in the human body. Tissues were prepared by carbon extraction and were examined first with an electron microprobe and later with a transmission electron microscope. Area diffraction patterns were obtained on some fibers to verify their identity as amphiboles. Uncoated fibers were characterized chemically and compared with standard reference asbestos samples. Standard ratio comparison indicated that the lung fibers from anthophyllite and amosite workers were depleted in magnesium, and that asbestos fibers from all lung tissues showed an increase in iron content. The authors conclude that the characteristic chemistry of uncoated asbestos fibers in the lungs of asbestos workers were comparable to standard reference fibers; however detectable, statistically significant chemical changes were observed.
NIOSH-Publication; NIOSH-Grant; Asbestos-mining; Asbestos-workers; Miners; Tissue-reactions; Histological-changes; Asbestos-dusts; Fibrous-dusts; Chemical-reactions; Chemical-analysis
Community Medicine MT Sinai School of Medicine Fifth Avenue and 100 Street New York, N Y 10029
17068-78-9; 12172-73-5; 12001-28-4
Journal of Histochemistry and Cytochemistry
Mount Sinai School of Medicine, New York, New York