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Use of Dyed Leaf in Studies on the Origin of Cotton Dust.
Morey-PR; Sasser-PE; Bethea-RM; Hersh-SP
Special Session on Cotton Dust, (Reprinted from Proceedings of the 1978 Beltwide Cotton Production-Mechanization Conference and Special Sessions, pages 97-104) 1979:97-104
A four part investigation was conducted to determine if fluorescently labeled, airborne particulate less than 10 microns in diameter could be collected when raw cotton containing dyed trash was processed through a card. Initial investigation results demonstrated that a selectively dyed botanical trash component (leaf blade) could be successfully added and blended into raw cotton, and that dispersion of a portion of this dyed botanical material into less than 10 micron elutriated particulate could be followed by epifluorescence microscopy. Results of the second part of the investigation indicated that the Vertical Elutriator, Area, and Anderson Samplers would provide comparable results in tracing the micronization of gross botanical trash into definite dust size classes. The third part of the research indicated that the leaf blade fractions collected in the Ro/Tap Shaker were less precisely defined in diameter than desired; and the fourth part demonstrated that the combined use of a Tyler RX-24 Portable Sieve Shaker and the Sonic Sifter isolated large amounts of dyed vegetable particulate of specific size. The investigation provided no a priori morphology that identified a dust particle as originating from leaf as opposed to all other possible sources of cotton dust particulate. The authors preferred this combined device method for studying the way that plant parts break up during mechanical processing.
NIOSH-Grant; Control-technology; Fluorometric-analysis; Analytical-methods; Particulate-dust; Microanalysis; Cotton-dust; Chemical-processing; Textile-workers; Textile-industry; Cotton-mills;
Biological Sciences Texas Tech University Post Office Box 4149 Lubbock, Ten 79409
Control Technology and Personal Protective Equipment; Research Tools and Approaches; Control-technology;
Special Session on Cotton Dust, (Reprinted from Proceedings of the 1978 Beltwide Cotton Production-Mechanization Conference and Special Sessions, pages 97-104)
Texas Tech University, Lubbock, Texas
Page last reviewed: September 2, 2020
Content source: National Institute for Occupational Safety and Health Education and Information Division