Proceedings: Third Special Session on Cotton Dust Research.
Proceedings of the Third Special Session on Cotton Dust Research, Beltwide Cotton Production Research Conference 1979:8-8
The relationship between the USDA Agricultural Marketing Service classification system for raw cotton grade and the amount of gram negative bacteria was investigated. Raw cotton samples were prepared and analyzed for botanical trash content. Samples were then weighed, suspended in normal saline, mixed by a vortex, and plated to permit colony counts of gram negative bacteria growing at 37 and 25 degrees-C, gram positive bacteria growing at 37 degrees-C, and thermophilic bacteria growing at 60 degrees-C. Endotoxin like activity was determined by the limulus polyphemus amebocyte lysate gelation reaction. Higher amounts of gram negative bacteria were found in raw cotton with more yellow color in grade divisions with more leaf. Geographical origin, field and harvesting conditions, and handling and processing of seed cotton were important factors in the amount of gram negative bacteria eventually found in raw cotton. Airborne gram negative bacteria content was associated with carding raw cotton from grade divisions with more leaf. More gram negative bacteria were found on the botanical trash in the raw cotton than on the cleaned lint itself. Attempts to correlate gram negative bacterial content and endotoxin levels were unsuccessful. The authors conclude that small amounts of botanical trash can contaminate massively a large amount of raw cotton lint.
NIOSH-Grant; Control-technology; Cotton-dust; Bacterial-dusts; Microanalysis; Analytical-methods; Biostatistics; Statistical-analysis;
Biological Sciences Texas Tech University Post Office Box 4149 Lubbock, Ten 79409
Control Technology and Personal Protective Equipment; Research Tools and Approaches; Control-technology;
Proceedings of the Third Special Session on Cotton Dust Research, Beltwide Cotton Production Research Conference
Texas Tech University, Lubbock, Texas