An in vivo method to prepare normal Schwann cells free of axons and myelin.
Spencer-PS; Weinberg-HJ; Krygier-Brevart-V; Zabrenetzky-V
Brain Res 1979 Apr; 165(1):119-126
A technique for preparing a viable population of Schwann cells based on the phenomenon of Wallerian degeneration was demonstrated. The sciatic nerves of albino-rabbits were exposed, crushed, and cut. The nerve stumps were then sewn into adjacent muscle surface to impede regenerative processes. Distal nerve stumps containing the proliferated Schwann cells were removed 5 to 12 weeks later, and morphologically studied by light and electron microscopy. Schwann cells formed longitudinal columns separated by endoneurial connective tissue. The authors conclude that this technique has the following advantages: simplicity and accessibility, large tissue yield, applicability to different species for comparative study, prolonged in-vivo viability and usefulness for analysis of axonal regulation of Schwann cell function and determination of the role of Schwann cell alteration in peripheral nerve reaction.
NIOSH-Publication; NIOSH-Grant; Neurotoxic-effects; Histology; Laboratory-animals; Nerve-fibers; Nerves-degeneration; Neurons; Cytological-analysis; Research-laboratories; In-vivo-study; Peripheral-nervous-system; Membrane-dysfunction; Nuclear-membrane
Pathology Albert Einstein Coll of Med 1300 Morris Park Avenue Bronx, N Y 10461
Neurotoxic Disorders; Neurotoxic-effects
Yeshiva University, New York, New York