Comparison of scanning transmission and backscattered electron imaging in the SEM, using heavy metal stained sections of biological tissue.
34th Annual Proceedings Electron Microscopy Society of America, Miami Beach, Florida, Bailey GW, ed. Baton Rouge, LA: Claitor's Publishing, 1976 Jan; :1-2
The results of a study which compared the scanning transmission electron microscope (STEM) and the backscattered electron (BSD) imaging in the scanning electron microscope (SEM) using embryonic chick tissue stained with uranyl-acetate (541093) and lead-citrate (14450603) are reported. It was determined that STEM was marginally adequate for sections of 60nm to 100nm and optimal at 200nm; however, above 200nm, resolution deteriorated and was uninformative. The BSE image was poor for sections of 60nm to 100nm and became optimal for thicknesses and 200nm and greater.
Analytical-methods; Analytical-instruments; Laboratory-equipment
34th Annual Proceedings Electron Microscopy Society of America, Miami Beach, Florida,