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Comparison of scanning transmission and backscattered electron imaging in the SEM, using heavy metal stained sections of biological tissue.
DeNee PB; Frederickson RG
34th Annual Proceedings Electron Microscopy Society of America, Miami Beach, Florida, Bailey GW, ed. Baton Rouge, LA: Claitor's Publishing, 1976 Jan; :1-2
The results of a study which compared the scanning transmission electron microscope (STEM) and the backscattered electron (BSD) imaging in the scanning electron microscope (SEM) using embryonic chick tissue stained with uranyl-acetate (541093) and lead-citrate (14450603) are reported. It was determined that STEM was marginally adequate for sections of 60nm to 100nm and optimal at 200nm; however, above 200nm, resolution deteriorated and was uninformative. The BSE image was poor for sections of 60nm to 100nm and became optimal for thicknesses and 200nm and greater.
Analytical-methods; Analytical-instruments; Laboratory-equipment
34th Annual Proceedings Electron Microscopy Society of America, Miami Beach, Florida,
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