Method Improvement for Thin Layer Chromatographic Separation and Fluorometric Determination of Benzo(a)pyrene.
NIOSH 1973 Dec:68 pages
A method improvement for thin layer chromatographic separation and fluorometric determination of benzo(a)pyrene (50328) is reported. The fluorescent detection procedure was studied with respect to the effects of sulfuric-acid (7664939) purity, light, air, and suspended particulates on the fluorescence of benzo(a)pyrene. Optimal fluorescence was obtained using ultrahigh purity sulfuric-acid purged with nitrogen monitoring for emission at 520nm. With respect to thin layer chromatographic separation of benzo(a)pyrene from coal- tar-pitch (8007452) volatile derivatives, alumina (1344281) coated aluminum (7429905) foil was shown to be the most useful support medium. Extraction of benzo(a)pyrene was best accomplished using a pentane (109660) methylene-chloride (75092) methanol (67561) solvent system. Chloroform (67663) and methylene-chloride were suggested as replacements for ether (60297) in the chromatography running solvents due to the problem of peroxide formation when ether is used. For levels of benzo(a)pyrene from 15 to 400ng, the standard deviation for this system of quantitation was determined to be from 3.5% to 6.2%. This is considered a significant improvement over the previous methodology.
NIOSH-Contract; Contract-099-73-0041; Carcinogenicity; Analytical-methods; Analytical-instruments; Structural-analysis; Chemical-analysis;
50-32-8; 7664-93-9; 8007-45-2; 1344-28-1; 7429-90-5; 109-66-0; 75-09-2; 67-56-1; 67-66-3; 60-29-7;
NTIS Accession No.
NIOSH, Cincinnati, Ohio, Final Report