Copper-binding activity of tobacco smoke condensate.
Michael-LW; Menden-EE; Petering-HG
Environ Sci Technol 1971 Mar; 5(3):249-251
The distribution of copper (7440508) between a buffered aqueous solution of the metal ion and an organic solution of tobacco smoke condensate (TSC) in 4-methyl-2-pentanone (MIBK) has been studied. The analytical parameters, metal ion and hydrogen ion concentrations, were investigated. Metal ion concentrations were determined by atomic absorption spectrophotometry. TSC was collected with use of a Mason Mark III Smoker and acetone traps maintained at 0 degrees centigrade. This material was transferred to MIBK by evaporation at reduced pressure. A 10-milliliter aliquot of an aqueous solution 0.1 molar in 2-amino-2-(hydroxymethyl)-1,3- propanediol (Tris) 0.01 molar in copper-chloride and pH 5.6 to 6.1 was equilibrated with an equal volume of the MIBK solution of TSC, which contained the equivalent of five cigarettes. Analysis of an aliquot from the organic phase after equilibration of the two phases yielded a maximum value of 30 micrograms of copper per cigarette when fresh, nonfractionated TSC was used. This simple, rapid extraction technique has been used to determine variations in the chemical activity of tobacco smoke condensate.
NIOSH-Publication; NIOSH-Grant; Grants-other; Analytical-methods; Tobacco-constituents; Smoking; Organic-solvents; Transition-metals; Chemical-binding
Environmental Health Kettering Laboratory College of Medicine Cincinnati, Ohio 45219
Other Occupational Concerns; Grants-other
Environmental Science and Technology
University of Cincinnati, Cincinnati, Ohio