Effect of protective agent against lipid-solvent-induced damages: ultrastructural and scanning electron microscopical study of human epidermis.
Lupulescu AP; Birmingham DJ
Arch Environ Health 1976 Feb; 31(1):33-36
The ultrastructural and relief (cytoarchitectural) changes of human epidermis following exposure to a protective gel and acetone (67641) or kerosene (8008206) were studied in 5 healthy volunteers. Topical applications of acetone and kerosene on unprotected skin produced cell damage and a disorganized pattern in the upper layers of epidermis. The ultrastructural changes following acetone consisted of large paranuclear vacuoles, swollen mitochondria, and clumped tonofilaments. Severe disorganization with intense cytolysis and enlarged intercellular spaces occurred after exposure to kerosene. Scanning electron microscopy revealed edematous cornified cells (corneocytes) following acetone exposure, whereas kerosene induced the occurrence of large lacunae in the swollen corneocytes. The application of a protective agent (50 percent water, 25 percent glycerin, 10 percent to 15 percent cellulose-methasol gum and 2 percent to 3 percent preservative) prior to solvent exposure substantially reduced the ultrastructural and relief changes of epidermal cells. It is not considered valid to extend these laboratory findings as field tests for workers in chemical factories without further studies from the physiological standpoint. (Grant No. R01-OH-00410)
NIOSH-Publication; NIOSH-Grant; Dermatitis; Cytopathology; Cell-alteration; Cell-morphology; Cytotoxicity; Histopathology; Skin-disorders; Skin-irritants; Organic-solvents; Fuels; Intracellular-alteration; Skin-protective-agents
Dermatology Wayne State University 540 East Canfield Street Detroit, Mich 48201
Archives of Environmental Health
Wayne State University, Detroit, Michigan