Cytogenetic evaluation of bone marrow cells in the rat following long-term inhalation exposure to nitrous oxide plus halothane.
NIOSH 1976 Jan; :1-24
The mutagenic potential of long-term exposure of 120 male Sprague- Dawley rats to nitrous-oxide (10024972) is measured by observing aberrations in bone marrow cell chromosomes. Forty rats serve as controls, 40 are exposed to 50ppm nitrous-oxide plus 1ppm halothane (151677), and 40 are exposed to 500ppm nitrous-oxide plus 10ppm halothane, for 7 hours a day, 5 days a week for 52 weeks. The most frequently found aberration is the chromatid gap. There is a significant increase in the number of gaps and this increase is dose- related. Chromatid breaks are the next most frequent aberrations observed. Chromosomal breaks are comparable among all groups. Chromosomal markers are significantly increased in the group receiving 500ppm nitrous-oxide. The incidence of polyploidy is comparable among control and treated groups. Fifty-nine percent of the animals in the first treatment group show aberrant cells; 68 percent in the second treatment group. It is concluded that exposure to 500ppm nitrous-oxide plus 10ppm halothane can cause chromosomal damage to bone marrow cells in the rat. (Contract No. 99-74-0046)
NIOSH-Publication; Chromosome-damage; Genetic-disorders; Toxic-gases; Toxic-substances; Drugs; Mutagens; Inhalation; Toxicity; Anesthetics; Contract-099-74-0046
Final Contract Report
NTIS Accession No.
National Institute for Occupational Safety and Health
Hazleton Laboratories America, Inc.