Histamine induction and release following proteolytic enzyme exposure.
Tolos WP; Richards DE; Scheel LD
Am Ind Hyg Assoc J 1975 Apr; 36(4):272-277
The mechanism of biologic response from exposure to a 12 percent subtilisin Carlsberg preparation is shown to be one of histamine release in the guinea pig. Three groups of guinea pigs were pretreated by intradermal injections with saline solution of the commercial proteolytic enzyme preparation containing 12 percent subtilisin Carlsberg, an alkaline protease preparation obtained from Aspergillus-oryzae that was isolated from cotton dust, or a nonproteolytic mixture of proteins and lipases obtained from cotton seeds. The histamine content of the lung, liver, and ear tissues of guinea pigs that were pretreated with any one of the three preparations showed an increase or induction of histamine when compared with the tissue histamine levels in untreated animals. Following challenge by intratracheal injection of a saline solution containing the subtilisin preparation, the guinea pigs pretreated with the same preparation showed a markedly reduced liver histamine level. Challenge by inhalation exposure to the subtilisin preparation of guinea pigs that were pretreated with any one of the above preparations resulted in a lower histamine concentration in the lungs and livers.
Synthetic-detergents; Biochemical-responses; Biological-monitoring; NIOSH-Author; Histamine-liberation; Enzyme-activity; Liver-function; Respiratory-system; Tissue-reactions; Sensitivity-testing; Cell-metabolism; Toxicology; Bioassays
American Industrial Hygiene Association Journal