An evaluation of the inhalation toxicity of one commercial proteolytic enzyme preparation.
Richards-DE; Scheel-LD; Groth-DH
Am Ind Hyg Assoc J 1975 Apr; 36(4):266-271
The inhalation toxicity of a commercial proteolytic enzyme preparation containing 12% subtilisin Carlsberg was studied in experimental animals. Guinea pigs that had been pretreated by a series of intradermal injections of the enzyme preparations at an air concentration of 41.2 milligrams per cubic meter, while normal guinea pigs and pretreated guinea pigs that were dosed with an antihistamine immediately prior to exposure survived. The 6-hour median lethal concentration for pretreated guinea pigs was determined as 24.7 milligrams per cubic meter. Normal rats, normal rabbits, and pretreated rabbits survived exposures to the enzyme preparation at concentrations as high as 36.8 milligrams per cubic meter. Pathologic examinations revealed changes only in the lungs of the exposed animals. These pulmonary alterations appear to be reversible. A histamine release is suggested as the primary effect of a secondary exposure to this proteolytic enzyme preparation.
Toxicology; Dosage; Histopathology; Respiratory-disorders; Lung-disorders; Histamine-liberation; Tissue-reactions; Synthetic-detergents; Housekeeping-products; Industrial-hygiene; Species-susceptibility; NIOSH-Author
American Industrial Hygiene Association Journal