Fractionation and chemical characterization studies on Micropolyspora faeni antigens.
Ann NY Acad Sci 1974 Feb; 221(1):199-204
Fractionation and chemical characterization studies on Micropolyspora faeni antigens are reported. Persons with hypersensitivity pneumonias such as farmer's lung usually show precipitating antibodies to antigens such as this. Two new isolation procedures were used. First the antigen mixture obtained by double dialysis was fractionated by ammonium-sulfate (7783202). Second, affinity chromatography using immobilized antibodies was employed. The chemical data on the 3 components fractionated with ammonium- sulfate are given (percent dryweight, percent amino acids, percent carbohydrate). The use of immobilized antibodies allows determination of inert as well as antigenically active material.
NIOSH-Publication; NIOSH-Grant; Pulmonary-system-disorders
Ronald C. Roberts, Marshfield Clinic Foundation for Medical Research and Education, Marshfield, Wisconsin 54449
Annals of the New York Academy of Sciences
Marshfield Clinic Fdn Med Res Education, Marshfield, Wisconsin