Specific metal staining of biological tissues for the SEM using backscatter electron or specimen current imaging.
Abraham JL; DeNee PB; Gelderman AH
NIOSH 1973 Aug; :1-4
A technique is described for distinguishing specific cell and tissue components with the Scanning Electron Microscope, combining differential impregnation of tissue by heavy elements with the backscatter mode of SEM imaging. Advantages of the method include large depth of field, surface morphology, histochemical identification of structures, and high resolution, with no special tissue processing required. Illustrations are presented to demonstrate the potential usefulness of the technique in investigations of coal workers' pneumoconiosis.
Analytical-methods; Electron-microscopy; Microscopy; Electron-optics; Heavy-metals; Biologic-tissues; Respiratory-system-disorders
Appalachian Laboratory for Occupational Respiratory Diseases, NIOSH (Reprint, Electronic Society of America, August, 1973), Morgantown, West Virginia, 4 pages, 5 references