Selective and non-selective histamine release from rat, guinea-pig, and human lung.
Comp Gen Pharmacol 1973 Mar; 4(13):81-90
The lungs of rats, guinea-pigs, and humans are compared for total histamine content, histamine release by compound 48/80 and Triton-X- 100, and histamine metabolism. Total lung histamine decreases in the following order: guinea-pig, human, rat. Under the experimental conditions used, the amount of histamine released by compound 48/80 decreases as follows: human, rat, guinea-pig. Both rat and guinea- pig metabolize added histamine, the former mainly via diamine oxidase, the latter by some other metabolic system and human lung does not metabolize added histamine. With guinea-pig and human lung, histamine release by compound 48/80 is found to be selective, since it is not accompanied by release of intracellular constituents such as lactic dehydrogenase, protein, and histamine metabolizing enzymes. With rat lung, compound 48/80 causes some nonselective histamine release. In the lungs of all three species, Triton-X-100 causes nonselective release of histamine.
NIOSH-Grant; Cytology; Allergenic-effects; Enzyme-inhibitors; Cellular-function; Stimulation; Enzyme-activity; Biochemistry;
Author Keywords: Rats; guinea-pigs; humans; lung; histamine; compound 48/80; Triton-X-100; selective release; non-selective release; metabolism; lactic dehydrogenase
John B Pierce Fdn Lab John B Pierce Fdn Lab 290 Congress Avenue New Haven, Conn 06519
Comparative and General Pharmacology
John B Pierce Fdn of Conn Inc, New Haven, Connecticut