Methods for a SEM study of coal workers' pneumoconiosis.
DeNee PB; Abraham JL; Gelderman AH
Scanning Electron Microscopy, Proceedings of Workshop on Scanning Electron Microscopy in Pathology, IIT Research Institute, Chicago, Illinois. 1973 Apr; (Pt III):411-418
This Scanning Electron Microscopy (SEM) study of lung tissue obtained from the National Coal Workers' Autopsy Study presents the first SEM demonstration of respirable dust in situ in the human lung. Several methods have been developed to yield valuable morphological information in the SEM from routine formalin-fixed, paraffin-embedded tissues. Correlation of the SEM image and the light microscopic (LM) images was made. Three cases selected to illustrate the methods represent a range of severity of coal workers' pneumoconiosis (CWP): (1) focal emphysema in bituminous coal miner, (2) reticular nodular lesions in a bituminous coal miner, (3) collagenous nodular lesions in a anthracite miner. There are no clearly demonstrable qualitative differences in the silica to silicate ratios among the three cases examined. However, there is a significant difference, by source excited X-ray fluorescence, in the elemental composition of the dust in Case No. 3 compared to Case No. 2.
Coal-miners; Cell-structure; Collagens; X-ray-fluorescence-analysis; Fibrosis; Pathology; Histochemistry; Microscopy;
Author Keywords: Coal; Dust; Pneumoconiosis; Emphysema; Fibrosis; Reticulin; Collagen; Scanning Electron Microscopy; Pathology; Silica; Histochemistry
Scanning Electron Microscopy, Proceedings of Workshop on Scanning Electron Microscopy in Pathology, IIT Research Institute, Chicago, Illinois