Collagen biosynthesis in rat lungs during exposure to asbestos.
Am Ind Hyg Assoc J 1971 Sep; 32(9):599-602
A cell free system capable of catalyzing both the incorporation of proline into a peptide chain and its subsequent hydroxylation to hydroxyproline has been obtained from rat lung tissue. These transformations are the key events in collagen biosynthesis, and their rate is controlled by the activity of the enzyme proline hydroxylase. Proline hydroxylase activity was measured as the hydroxyproline: proline ratio after incubation of lung extract with C-14- proline. In preparations obtained from animals which received intratracheal injections of crocidolite dust, the ratio was elevated, indicating that tissue reaction to asbestos dust apparently commences with stimulation of collagen biosynthesis. In preparations obtained from animals exposed to asbestos dust by prolonged inhalation, the ratio was depressed. These animals had advanced fibrotic responses in their lungs.
CIS; NIOSH-Publication; NIOSH-Grant; Organic-compounds; Enzymes; Asbestos-dust; Dust-inhalation; Bioassays; Fibrosis; Collagen-biosynthesis
Occupational & Environ Health Wayne State University 625 Mullett Detroit, Mich 48226
American Industrial Hygiene Association Journal
Wayne State University, Detroit, Michigan