Ion and macromolecular transport in the alveolar macrophage.
Robin ED; Smith JD; Tanser AR; Adamson JS; Millen JE; Packer B
Biochim Biophys Acta 1971 Jul; 241(1):117-128
Some aspects of sodium, potassium, and chloride ion transport and macromolecular transport have been studied in isolated rabbit alveolar macrophages. The steady-state intracellular concentrations in Ringer's solution are presented for the three ions in terms of milliequivalents per kilogram cell water. The two rate constants, rapid phase and slow phase, are given for the sodium ion efflux. It appears that this cell has high permeability for sodium ion and the high leak down the electrochemical gradient requires a high rate of active transport. Classical relatively high molecular weight extracellular markers are excluded from cell water; however, ferritin (molecular weight 465,000) influx is rapid with intracellular/extracellular concentrations greater than 1.0 within 3 minutes. Simultaneous exposure of cells to both ferritin and dextran leads to no measurable increase in cellular dextran uptake suggesting high specificity for ferritin uptake.
NIOSH-Publication; NIOSH-Grant; Cytology; Biophysical-effects; Cell-mechanics; Pulmonary-functions; Phagocytosis; Ion-exchange; PM9004540; Biochemical-effects; PM9007732
Dr. Eugene D. Robin, Department of Medicine, Stanford University Medical Center, Stanford, Calif. 94305, U.S.A
7778-80-5; 10233-01-9; 7646-93-7; 7647-14-5
Biochimica et Biophysica Acta
Stanford University, Stanford, California