A Projectile Concussive Impact Model Produces Neuroinflammation in Both Mild and Moderate-Severe Traumatic Brain Injury
Updated May 9, 2023
NIOSH Dataset RD-1066-2023-0
Traumatic brain injury (TBI) is as a major cause of death and disability experienced by nearly 3 million people annually resulting from falls, vehicular accidents, or from being struck by or against an object. While TBIs can range in severity, the majority of injuries are considered to be mild. However, TBI of any severity has the potential to have long-lasting neurological effects including headaches, cognitive/memory impairments, mood dysfunction, and fatigue as a result of neuronal damage and neuroinflammation. The goal of the study was to evaluate the neuroinflammatory and neuronal damage outcomes associated with mild or moderate-severe TBI via the modification of an established closed-head injury model of TBI by varying the material of the projectile. Rats that received TBI using a stainless steel projectile exhibited outcomes strongly correlated to moderate-severe TBI, such as prolonged unconsciousness, impaired neurobehavior, increased risk for hematoma and death, as well as significant neuronal degeneration and neuroinflammation throughout the cortex, hippocampus, thalamus, and cerebellum. In contrast, rats that received TBI with an aluminum projectile exhibited characteristics more congruous with mild TBI, such as a trend for longer periods of unconscious in the absence of neurobehavioral deficits, a lack of neurodegeneration and mild neuroinflammation. Our results indicate that different levels of behavioral, neuroinflammatory, and damage outcomes are associated with differing levels of TBI severity.
- TBI Behavioral Evaluation [XLS – 784 B]
- TBI PCR Cerebellum [XLS – 779 B]
- TBI PCR Hippocampus [XLS – 779 B]
- TBI PCR Left Frontal Cortex [XLS – 765 B]
- TBI PCR Left Middle Cortex [XLS – 765 B]
- TBI PCR Left Rear Cortex [XLS – 764 B]
- TBI PCR Right Frontal Cortex [XLS – 760 B]
- TBI PCR Right Middle Cortex [XLS – 765 B]
- TBI PCR Right Rear Cortex [XLS – 765 B]
- TBI PCR Striatum [XLS – 779 B]
- TBI Data Dictionary [PDF – 125 KB]
- TBI Pilot Detailed Data Collection Methods [PDF – 146 KB]
Data Collection Methods
Adult male Sprague-Dawey rats received either a mild or moderate-severe TBI using the projectile concussive impact model of TBI with an aluminum or stainless steel ball bearing, respectively. Rats were evaluated for the duration of unconsciousness following TBI, as well as neurobehavioral dysfunction from 1 to 72 hours post-TBI. Inflammatory cytokine mRNA expression was measured in the brain by quantitative real time PCR at 6 hours post-TBI.
Michalovicz LT, Kelly KA, Craddock TJA, O’Callaghan JP (2023) A Projectile Concussive Impact Model Produces Neuroinflammation in Both Mild and Moderate-Severe Traumatic Brain Injury Brain Sciences. 2023, 13, 623. https://doi.org/10.3390/brainsci13040623.
The work that generated this dataset was supported by Intramural funding from the Centers for Disease Control and Prevention – National Institute for Occupational Safety and Health and Congressionally-Directed Medical Research Program, Gulf War Illness Research Program grants: GW120037 for the Boston University Gulf War Illness Consortium (K. Sullivan, PI) and GW180106 (LT. Michalovicz, PI).
When a publication makes use of this dataset, acknowledgement of the development of the dataset should be attributed to Michalovicz LT, Kelly KA, O’Callaghan JP.
NIOSH/HELD Toxicology and Molecular Biology Branch