Zinc sulfate (1:1)

RTECS #
ZH5260000
CAS #
7733-02-0
See: NMAM or OSHA Methods
Updated
March 2019
Molecular Weight
161.43
Molecular Formula
O4S·Zn
Synonyms
Bonazen
Bufopto zinc sulfate
Complexonat
OP-Thal-zin
Optraex
Sulfate de zinc (French)
Sulfuric acid, zinc salt (1:1)
Verazinc
White copperas
White vitriol
Zinc sulphate
Zinc vitriol
Zincomed
Zinkosite

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 420 µg moderateJAPMA8 45,474,1956

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic AnalysisAscites tumor/rat1 gm/kgGANNA2 54,155,1963
DNA inhibitionHeLa cell/human1 µmol/L/4HBECTA6 32,220,1984
gene conversion and mitotic recombination/Saccharomyes cerevisiae100 mmol/LMUREAV 117,149,1983
micronucleus testintraperitoneal/mouse1800 µg/kgGWZHEW 12,77,1986
morphological transformembryo/hamster200 µmol/LCNREA8 39,193,1979
mutation in microorganisms/Saccharomyes cerevisiae100 mmol/L (-enzymatic activation step)MUREAV 117,149,1983

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
intravaginal/rabbit 34573 µg/kg (1D prior to copulation)Reproductive: Effects on fertility: Female fertility index (e.g., # females pregnant per # sperm positive females; # females pregnant per # females mated)CCPTAY 22,659,1980
intravenous/hamster 2 mg/kg (8D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)ENVRAL 6,95,1973
oral/domestic animal 193 gm/kg (6-20W pregnant)Reproductive: Effects on fertility: Abortion

Reproductive: Effects on newborn: Live birth index (Litter size (e.g., # fetuses per litter; measured after birth)

Reproductive: Effects on newborn: Weaning or lactation index (e.g., # alive at weaning per # alive at day 4)
ENVRAL 20,1,1979
oral/rat 333 mg/kg (1-18D pregnant)Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)NURIBL 13,33,1976
subcutaneous/hamster 15 mg/kg (8D pregnant)Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)

Reproductive: Specific developmental abnormalities: Cardiovascular (circulatory) system
ENVRAL 35,405,1984

Tumorigenic Data and References

Route/OrganismDoseEffectReference
subcutaneous/rabbit lowest published toxic dose: 3625 µg/kg/5D- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Tumorigenic: Tumors at site of application
COREAF 236,1387,1953

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Human, colon tumor Inhibitor Concentration Low: 200 µmol/L/24HIn Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.TIVIEQ 27,834,2013
In Vitro/Human, colon tumor Inhibitor Concentration Low: 200 µmol/L/6HIn Vitro Toxicity Studies: Other assaysTIVIEQ 27,834,2013
In Vitro/Human, fibroblast Inhibitor Concentration Low: 2.5 mg/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TOLED5 207,197,2011
In Vitro/Human, leukemia cells Inhibitor Concentration (25 percent kill): 670.8 mg/L/24HIn Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc.TIVIEQ 26,1150,2012
In Vitro/Human, leukemia cells Inhibitor Concentration (5 percent kill): 160 mg/L/30MIn Vitro Toxicity Studies: Cell countingTIVIEQ 27,857,2013
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 90 µmol/L/48HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.JIBIDJ 164,59,2016
In Vitro/okaInhibitor Concentration Low: 100 µmol/L/24HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)

In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.

In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.
TIVIEQ 27,834,2013
In Vitro/okaInhibitor Concentration Low: 200 µmol/L/6HIn Vitro Toxicity Studies: Other assaysTIVIEQ 27,834,2013
In Vitro/okaInhibitor Concentration (50 percent kill): 198.89 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 44,392,2017
In Vitro/okaInhibitor Concentration Low: 1000 µmol/L/4HIn Vitro Toxicity Studies: Apoptosis in vitroTIVIEQ 44,392,2017
intraduodenal/Dog lowest published toxic dose: 161 mg/kg/1HGastrointestinal: Alteration in gastric secretionAJPHAP 248,G28,1985
intraperitoneal/mouse lethal dose (50 percent kill): 29060 µg/kgCOREAF 256,1043,1963
intraperitoneal/rat lethal dose (50 percent kill): 196 mg/kgINJPD2 23,153,1991
intraperitoneal/rat lowest published toxic dose: 32.3 gm/kgLiver: Other changes

Biochemical: Metabolism (intermediary): Other proteins
TXCYAC 213.74.2005
intraperitoneal/rat lowest published toxic dose: 15 mg/kgLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
TOXID9 -,239,2009
intratracheal/rat lowest published toxic dose: 322.86 µg/kgCardiac: Other changes

Nutritional and Gross Metabolic: Changes in: Other metals

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
JTEHF8 69,2011,2006
intratracheal/rat lowest published toxic dose: 33 µg/kgLung, Thorax, or Respiration: Sputum

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TOSCF2 70,73,2002
intratracheal/rat lowest published toxic dose: 161 µg/kgCardiac: Other changes

Lung, Thorax, or Respiration: Other changes
TOXID9 78,17,2004
intratracheal/rat lowest published toxic dose: 161 µg/kgCardiac: Cardiomyopathy including infarctionTOXID9 78,17,2004
intratracheal/rat lowest published toxic dose: 131 µg/kgCardiac: Other changes

Lung, Thorax, or Respiration: Acute pulmonary edema

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 211,41,2006
intravenous/Dog lowest published toxic dose: 4.8 mg/kg/15MGastrointestinal: Alteration in gastric secretionAJPHAP 248,G28,1985
intravenous/Dog lowest published lethal dose: 66 mg/kgEQSSDX 1,1,1975
intravenous/mouse lethal dose (50 percent kill): 23300 µg/kgINJPD2 23,153,1991
intravenous/rabbit lowest published lethal dose: 23 mg/kgTXAPA9 9,269,1966
intravenous/rat lethal dose (50 percent kill): 69900 µg/kgINJPD2 23,153,1991
oral/human lowest published toxic dose: 45 mg/kg/7D- continuousCardiac: Pulse rate increased without fall in BP

Gastrointestinal: Other changes

Blood: Normocytic anemia
BMJOAE 1,754,1978
oral/human lowest published toxic dose: 106 mg/kgVascular: BP lowering not characterized in autonomic section

Lung, Thorax, or Respiration: Acute pulmonary edema

Gastrointestinal: Hypermotility, diarrhea
BMJOAE 1,1390,1977
oral/human lowest published toxic dose: 14.29 mg/kgGastrointestinal: Nausea or vomitingHBPTO* 2,1366,2001
oral/man lowest published toxic dose: 180 mg/kg/6W- intermittentBlood: Other changesJAMAAP 252,1443,1984
oral/mouse lethal dose (50 percent kill): 245 mg/kgYHTPAD 22,230,1987
oral/rabbit lethal dose (50 percent kill): 2 gm/kgIPSTB3 3,93,1976
oral/woman lowest published toxic dose: 3120 mg/kg/43W- intermittentBlood: Microcytosis with or without anemia

Blood: Angranulocytosis

Nutritional and Gross Metabolic: Changes in: Other metals
GASTAB 94,508,1988
subcutaneous/Dog lowest published lethal dose: 78 mg/kgEQSSDX 1,1,1975
subcutaneous/mouse lethal dose (50 percent kill): 781 mg/kgGastrointestinal: Changes in pancreatic weight

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes
JJTHEC 44,305,1998
subcutaneous/rabbit lowest published lethal dose: 300 mg/kgEQSSDX 1,1,1975
subcutaneous/rat lowest published lethal dose: 330 mg/kgEQSSDX 1,1,1975

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
inhalation/rat lowest published toxic concentration: 100 µg/m3/16W- intermittentCardiac: Cardiomyopathy including infarction

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other oxidoreductases

Biochemical: Metabolism (intermediary): Effect on mitochondrial function
TXAPA9 232,69,2008
intraperitoneal/rat lowest published toxic dose: 84 mg/kg/4W- intermittentEndocrine: Evidence of thyroid hyperfunction

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)
TXCYAC 195,69,2004
intratracheal/rat lowest published toxic dose: 267.2 µg/kg/8W- intermittentCardiac: Other changes

Lung, Thorax, or Respiration: Other changes

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TOXID9 44,89,2005
intratracheal/rat lowest published toxic dose: 267.2 µg/kg/16W- intermittentCardiac: Other changes

Lung, Thorax, or Respiration: Other changes

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TOXID9 44,89,2005
oral/domestic animal lowest published toxic dose: 7110 mg/kg/4W- intermittentGastrointestinal: Changes in structure or function of exocrine pancreas

Gastrointestinal: Hypermotility, diarrhea

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
JJATDK 4,92,1984
oral/human lowest published toxic dose: 560 mg/kg/8W- intermittentGastrointestinal: Nausea or vomitingBJDEAZ 146,423,2002
oral/mouse lowest published toxic dose: 36 mg/kg/10D- intermittentBehavioral: Food intake (animal)

Nutritional and Gross Metabolic: Other changes

Biochemical: Metabolism (intermediary): Other proteins
CEXPB9 32,273,2005
oral/mouse lowest published toxic dose: 44424 mg/kg/18D- continuousGastrointestinal: Changes in structure or function of exocrine pancreas

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other enzymes
TXCYAC 132,33,1999
oral/rat lowest published toxic dose: 2889 mg/kg/8W- continuousLiver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Plasma proteins not involving coagulation
DCTODJ 1,11,2006
oral/rat lowest published toxic dose: 476.7 mg/kg/3W- intermittentLiver: Other changes

Biochemical: Metabolism (intermediary): Lipids including transport
CEXPB9 27,694,2000
oral/rat lowest published toxic dose: 74 mg/kg/3D- intermittentMusculoskeletal: Other changes

Nutritional and Gross Metabolic: Changes in: Ca
TXAPA9 67,224,1983
oral/rat lowest published toxic dose: 1200 mg/kg/60D- continuousLiver: Other changes

Nutritional and Gross Metabolic: Changes in: Other metals
CHHTAT 72,391,1992
subcutaneous/mouse lowest published toxic dose: 40 mg/kg/2D- intermittentKidney, Ureter, and Bladder: Other changes in urine composition

Kidney, Ureter, and Bladder: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)
TXAPA9 204,9,2005
subcutaneous/mouse lowest published toxic dose: 96858 µg/kg/2D- intermittentCardiac: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Cytochrome oxidases (indlucing oxidative phosphrylation)
TOLED5 170,66,2007
subcutaneous/rat lowest published toxic dose: 200 mg/kg/10D- intermittentLiver: Other changesTOLED5 158,108,2005

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEWFOREAE 7,313,1942
TOXICOLOGY REVIEWENTOX* -,479,2005
TOXICOLOGY REVIEWHBTME* ,925,2007
TOXICOLOGY REVIEWHUTOX* -,515,1996
TOXICOLOGY REVIEWMUREAV 721,27,2011
TOXICOLOGY REVIEWFCTOD7 109,771,2017
TOXICOLOGY REVIEWRTOPDW 54,294,2009

Standards and Regulations

OrganizationStandardReference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATIONFEREAC 54,7740,1989
Occupational Exposure Limit-GERMANYMAK 0.1 mg(Zn)/m3, resp, 2011
Occupational Exposure Limit-GERMANYMAK 2 mg(Zn)/m3, inhal, 2011
Occupational Exposure Limit-SWITZERLANDMAK-week 0.1 mg(Cu)/m3, KZG-week 0.2 mg(Cu)/m3, inhal, JAN2011
Occupational Exposure Limit-SWITZERLANDMAK-week 0.1 mg(Zn)/m3, KZG-week 0.4 mg(Zn)/m3, resp, JAN2011
Occupational Exposure Limit-SWITZERLANDMAK-week 2 mg(Zn)/m3, KZG-week 4 mg(Zn)/m3, inhal, JAN2011

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code 77220; Number of Industries 52; Total Number of Facilities 4755; Number of Occupations 66; Total Number of Employees Exposed 89451; Total Number of Female Employees Exposed 27887
National Occupational Hazard Survey 1974Hazard Code 77220; Number of Industries 52; Total Number of Facilities 3067; Number of Occupations 47; Total Number of Employees Exposed 40653

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-SA7/SHE; S cerevisiae gene conversion
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
Page last reviewed: November 16, 2018