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Registry of Toxic Effects of Chemical Substances (RTECS)

o-Toluidine

RTECS #

XU2975000

CAS #

95-53-4

Updated

April 2017

Molecular Weight

107.17

Molecular Formula

C7H9N

Synonyms

1-Amino-2-methylbenzene
1-Methyl-2-aminobenzene
2-Amino-1-methylbenzene
2-Aminotoluene
2-Methyl-1-aminobenzene
2-Methylaniline
2-Methylbenzenamine
2-Toluidine
Aniline, 2-methyl-
Benzenamine, 2-methyl- (9CI)
C.I. 37077
o-Aminotoluene
o-Methylaniline
o-Methylbenzenamine
ortho-Toluidine
o-Toluidin (Czech)
o-Toluidine (ACGIH:OSHA)
o-Toluidyna (Polish)
o-Tolylamine
RCRA waste number U328

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
eye /rabbit 750 µg/24H severe85JCAE -,460,1986
skin /rabbit 10 mg/24H open irritation test mildAIHAAP 23,95,1962
skin /rabbit 500 mg/24H mild85JCAE -,460,1986

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
body fluid assaySalmonella typhimurium/rat300 mg/kgMUREAV 79,173,1980
body fluid assayovary/rat400 mg/kgMUREAV 143,263,1985
Cytogenetic Analysisliver/rat175 mg/LPMRSDJ 5,387,1985
Cytogenetic Analysisliver/hamster12 mg/LPMRSDJ 5,397,1985
Cytogenetic Analysislung/hamster1 gm/LPMRSDJ 5,427,1985
Cytogenetic Analysisovary/hamster250 mg/LPMRSDJ 5,413,1985
Cytogenetic Analysisintraperitoneal/mouse45 mg/kgHPV300 -,-,2001
DNA Damageintraperitoneal/mouse100 mg/kgHPV300 -,-,2001
DNA Damageoral/rat500 mg/kgHPV300 -,-,2001
DNA Damageovary/hamster20 mmol/LPMRSDJ 5,367,1985
DNA Damageother cell types/rat8 mmol/L/20HTXCYAC 179,115,2002
DNA Damageother cell types/human16 mmol/L/20HTXCYAC 179,115,2002
DNA Damageliver/rat319 mg/LPMRSDJ 5,353,1985
DNA Damageoral/mouse600 mg/kgMUREAV 440,1,1999
DNA inhibitionintraperitoneal/mouse20 gm/kgARGEAR 51,605,1981
DNA inhibitionHeLa cell/human4 mmol/LCRNGDP 13,2389,1992
DNA inhibitionoral/mouse200 mg/kgHPV300 -,-,2001
DNA repair/Escherichia coli20 µL/plateJJIND8 62,873,1979
gene conversion and mitotic recombination/Saccharomyes cerevisiae378 mg/LPMRSDJ 5,271,1985
micronucleus testmultiple/non-mammalian species4 ppmMUREAV 292,83,1993
micronucleus testlymphocyte/human2 mmol/LMUREAV 291,93,1993
micronucleus testintraperitoneal/rat300 mg/kgMUREAV 583,133,2005
micronucleus testoral/rat600 mg/kgMUREAV 583,133,2005
morphological transformembryo/mouse500 mg/LPMRSDJ 5,659,1985
morphological transformembryo/hamster100 mg/LPMRSDJ 5,665,1985
morphological transformfibroblast/mouse300 mg/L/10DMUREAV 702,100,2010
mutation in mammalian somatic cellslymphocyte/mouse10 mg/LPMRSDJ 5,587,1985
mutation in mammalian somatic cellslymphocyte/human450 mg/LPMRSDJ 5,497,1985
mutation in microorganisms/Saccharomyes cerevisiae378 mg/L (+/-enzymatic activation step)PMRSDJ 5,271,1985
mutation in microorganismslymphocyte/human300 mg/L (+enzymatic activation step)PMRSDJ 5,497,1985
mutation in microorganisms/Salmonella typhimurium40 µg/plate (-enzymatic activation step)PJABDW 53,34,1977
mutation in microorganisms/Salmonella typhimurium250 ng/plate (+enzymatic activation step)AGSREJ 13,13,1995
mutation in microorganismslymphocyte/mouse12500 µg/L (+enzymatic activation step)PMRSDJ 5,525,1985
mutation in microorganismsembryo/mouse250 mg/L (+enzymatic activation step)PMRSDJ 5,639,1985
phage inhibition capacity/Escherichia coli100 mmol/LMDMIAZ 31,11,1979
sex chromosome loss and nondisjunctionliver/hamster60 mg/LPMRSDJ 5,397,1985
sister chromatid exchangelymphocyte/human200 µmol/LMUREAV 211,77,1989
sister chromatid exchangeliver/rat21800 µg/LPMRSDJ 5,387,1985
sister chromatid exchangeintraperitoneal/hamster200 mg/kgMUREAV 113,33,1983
sister chromatid exchangeoral/hamster200 mg/kgMUREAV 113,33,1983
sister chromatid exchangeovary/hamster125 mg/LPMRSDJ 5,451,1985
sister chromatid exchangelung/hamster2500 µmol/LPMRSDJ 5,469,1985
specific locus testoral/Drosophila melanogaster1 mmol/LPMRSDJ 5,313,1985
Unscheduled DNA SynthesisHeLa cell/human50 µL/LPMRSDJ 5,347,1985
Unscheduled DNA Synthesisliver/rat1 mmol/LPMRSDJ 5,371,1985

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
skin/rat 9520 mg/kg (17W male)Reproductive: Paternal effects: Spermatogenesis (including genetic material, sperm morphology, motility, and count)

Reproductive: Effects on newborn: Physical
GTPZAB 27(9),47,1983
skin/rat 9520 mg/kg (17W prior to copulation)Reproductive: Maternal effects: Menstrual cycle changes or disorders

Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)

Reproductive: Effects on newborn: Physical
GTPZAB 27(9),47,1983
skin/rat 952 mg/kg (17W prior to copulation)Reproductive: Maternal effects: Ovaries, fallopian tubes

Reproductive: Maternal effects: Menstrual cycle changes or disorders
GTPZAB 27(9),47,1983

Tumorigenic Data and References

Route/OrganismDoseEffectReference
oral/rat lowest published toxic dose: 109 gm/kg/2Y- continuousTumorigenic: Neoplastic by RTECS criteria

Blood: Tumors
FCTOD7 25,619,1987
oral/rat toxic dose: 7250 mg/kg/23W- continuousTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors
APMIAL 26,447,1949
subcutaneous/mouse lowest published toxic dose: 320 mg/kg (15-21D pregnant)Tumorigenic: Neoplastic by RTECS criteria

Lung, Thorax, or Respiration: Tumors

Reproductive: Tumorigenic effects: Transplacental tumorigenesis
BEXBAN 78,1402,1974
subcutaneous/rabbit lowest published toxic dose: 840 mg/kg/14W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Kidney, Ureter, and Bladder: Bladder tumors
IMEMDT 27,155,1982

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
inhalation/man lowest published toxic concentration: 25 mg/m3Kidney, Ureter, and Bladder: Urine volume increased

Kidney, Ureter, and Bladder: Hematuria

Blood: Methemoglobinemia- Carboxhemoglobinemia
GTPZAB 14(8),7,1970
inhalation/rat lethal concentration (50 percent kill): 862 ppm/4HBehavioral: Somnolence (general depressed activity)

Behavioral: Tremor

Lung, Thorax, or Respiration: Cyanosis
NTIS** OTS0570956
intraperitoneal/mouse lethal dose (50 percent kill): 113 mg/kgHPV300 -,-,2001
oral/cat lethal dose (50 percent kill): >50 mg/kgHPV300 -,-,2001
oral/cat lowest published lethal dose: 150 mg/kgBehavioral: Coma

Lung, Thorax, or Respiration: Dyspnea

Blood: Methemoglobinemia- Carboxhemoglobinemia
XPHBAO 271,49,1941
oral/frog lowest published lethal dose: 151 mg/kgBehavioral: Coma

Lung, Thorax, or Respiration: Dyspnea

Blood: Methemoglobinemia- Carboxhemoglobinemia
XPHBAO 271,49,1941
oral/mammal (species unspecified) lethal dose (50 percent kill): 1700 mg/kgGTPZAB 32(10),25,1988
oral/mouse lethal dose (50 percent kill): 520 mg/kgBlood: Normocytic anemia

Blood: Pigmented or nucleated red blood cells

Blood: Methemoglobinemia- Carboxhemoglobinemia
IMEMDT 27,155,1982
oral/mouse lethal dose (50 percent kill): 520 mg/kgHPV300 -,-,2001
oral/rabbit lethal dose (50 percent kill): 840 mg/kgHPV300 -,-,2001
oral/rabbit lethal dose (50 percent kill): 840 mg/kgBlood: Normocytic anemia

Blood: Pigmented or nucleated red blood cells

Blood: Methemoglobinemia- Carboxhemoglobinemia
IMEMDT 27,155,1982
oral/rat lethal dose (50 percent kill): 670 mg/kgBlood: Normocytic anemia

Blood: Pigmented or nucleated red blood cells

Blood: Methemoglobinemia- Carboxhemoglobinemia
IMEMDT 27,155,1982
oral/rat lowest published toxic dose: 60 mg/kgLiver: Other changes

Kidney, Ureter, and Bladder: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
TXCYAC 186,67,2003
oral/rat lethal dose (50 percent kill): 635 mg/kgHPV300 -,-,2001
skin/rabbit lethal dose (50 percent kill): 3250 µL/kgAIHAAP 23,95,1962
skin/rabbit lethal dose (50 percent kill): 3250 mg/kgHPV300 -,-,2001
subcutaneous/rat lethal dose (50 percent kill): 1115 mg/kgHPV300 -,-,2001

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
intraperitoneal/rat lowest published toxic dose: 225 mg/kg/3D- intermittentLiver: Other changes

Kidney, Ureter, and Bladder: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
HPV300 -,-,2001
oral/rat lowest published toxic dose: 131040 mg/kg/104W- intermittentLiver: Other changes

Kidney, Ureter, and Bladder: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
HPV300 -,-,2001
oral/rat lowest published toxic dose: 1125 mg/kg/5D- continuousBlood: Changes in spleen

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
FAATDF 3,285,1983
oral/rat lowest published toxic dose: 10200 mg/kg/30D- intermittentKidney, Ureter, and Bladder: Proteinuria

Blood: Normocytic anemia

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
VINIT* #4035-77
oral/rat lowest published toxic dose: 1125 mg/kg/5D- intermittentBlood: Methemoglobinemia- Carboxhemoglobinemia

Blood: Changes in spleen
CICAD* VI:7,1,1998
oral/rat lowest published toxic dose: 1148 mg/kg/4W- continuousLiver: Other changes

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
IJTOFN 24,365,2005
oral/rat lowest published toxic dose: 2086 mg/kg/2W- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gainIJTOFN 24,365,2005
oral/rat lowest published toxic dose: 2190 mg/kg/30D- continuousBlood: Methemoglobinemia- CarboxhemoglobinemiaIJTOFN 24,365,2005
oral/rat lowest published toxic dose: 4004 mg/kg/91D- continuousBlood: Methemoglobinemia- CarboxhemoglobinemiaIJTOFN 24,365,2005

Reviews

OrganizationStandardReference
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-time-weighted average 2 ppm (skin)DTLVS* TLV/BEI,2013
American Conference of Governmental Industrial Hygienists (ACGIH)Threshold Limit Value-Confirmed animal carcinogenDTLVS* TLV/BEI,2013
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 99,451,2010
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 100F,99,2012
International Agency for Research on Cancer (IARC)Cancer Review:Animal Sufficient EvidenceIMEMDT 77,564,2000
International Agency for Research on Cancer (IARC)Cancer Review:Human Limited EvidenceIMEMDT 77,564,2000
International Agency for Research on Cancer (IARC)Cancer Review:Human Sufficient EvidenceIMEMDT 99,451,2010
International Agency for Research on Cancer (IARC)Cancer Review:Human Sufficient EvidenceIMEMDT 100F,99,2012
International Agency for Research on Cancer (IARC)Cancer Review:Group 2AIMEMDT 77,564,2000
International Agency for Research on Cancer (IARC)Cancer Review:Group 1IMEMDT 100F,99,2012
International Agency for Research on Cancer (IARC)Cancer Review:Group 1IMEMDT 99,451,2010
TOXICOLOGY REVIEWMUREAV 543,201,2003
TOXICOLOGY REVIEWMUREAV 567,109,2004
TOXICOLOGY REVIEWTCMUD8 23(Suppl 1),187,2003
TOXICOLOGY REVIEWTXCYAC 196,1,2004
TOXICOLOGY REVIEWMUREAV 588,88,2005
TOXICOLOGY REVIEWFCTOD7 44,1699,2006
TOXICOLOGY REVIEWMUREAV 627,10,2007
TOXICOLOGY REVIEWENTOX* -,104,2005
TOXICOLOGY REVIEWMUREAV 627,59,2007
TOXICOLOGY REVIEWENTOX* -,206,2005
TOXICOLOGY REVIEWBCLPT* 96,131,2005
TOXICOLOGY REVIEWMUREAV 584,1,2005
TOXICOLOGY REVIEWMUREAV 654,114,2008
TOXICOLOGY REVIEWMUTAEX 23,153,2008
TOXICOLOGY REVIEWMUREAV 751,49,2012
TOXICOLOGY REVIEWMUREAV 752,99,2013
TOXICOLOGY REVIEWMUREAV 769,34,2014
TOXICOLOGY REVIEWMUREAV 768,27,2016
TOXICOLOGY REVIEWMUREAV 802,1,2016

Standards and Regulations

OrganizationStandardReference
Mine Safety and Health Administration (MSHA) STANDARD-airtime-weighted average 5 ppm (22 mg/m3)DTLVS* 3,261,1971
Occupational Exposure Limit IN ARGENTINA, BULGARIA, COLOMBIA, JORDAN check ACGIH TLV;
Occupational Exposure Limit IN SINGAPORE, VIETNAM check ACGIH TLV
Occupational Exposure Limit-AUSTRALIAtime-weighted average 2 ppm (8.8 mg/m3), Carcinogen, JUL2008
Occupational Exposure Limit-AUSTRIATRK-TMW 0.1 ppm (0.5 mg/m3);KZW 0.4 ppm (2 mg/m3), skin, 2007
Occupational Exposure Limit-BELGIUMtime-weighted average 2 ppm (8.9 mg/m3), Skin, Carcinogen, MAR2002
Occupational Exposure Limit-DENMARKtime-weighted average 2 ppm (9 mg/m3), skin, carc, MAY2011
Occupational Exposure Limit-FINLANDtime-weighted average 2 ppm, short term exposure limit 4 ppm, skin, NOV2011
Occupational Exposure Limit-FRANCEVME 2 ppm (9 mg/m3), C2 Carcinogen, FEB2006
Occupational Exposure Limit-HUNGARYceiling concentration 0.5 mg/m3, Skin, Carcinogen, SEP2000
Occupational Exposure Limit-ICELANDtime-weighted average 2 ppm (9 mg/m3), carc, skin, NOV2011
Occupational Exposure Limit-JAPANOccupational Exposure Limit 1 ppm (4.4 mg/m3), skin, 2A carc, MAY2012
Occupational Exposure Limit-KOREAtime-weighted average 2 ppm (9 mg/m3), carcinogen, skin, 2006
Occupational Exposure Limit-MEXICOtime-weighted average 5 ppm (22 mg/m3) (skin), 2004
Occupational Exposure Limit-NEW ZEALANDtime-weighted average 0.2 ppm (0.89 mg/m3), skin, JAN2002
Occupational Exposure Limit-NORWAYtime-weighted average 1 ppm (4.5 mg/m3), JAN1999
Occupational Exposure Limit-PERUtime-weighted average 2 ppm (8.8 mg/m3), JUL2005
Occupational Exposure Limit-POLANDMAC(time-weighted average) 3 mg/m3, MAC(short term exposure limit) 9 mg/m3, JAN1999
Occupational Exposure Limit-RUSSIAtime-weighted average 0.5 mg/m3, short term exposure limit 1 mg/m3, Skin, JUN2003
Occupational Exposure Limit-SWITZERLANDMAK-week 0.1 ppm (0.5 mg/m3), carc 2, skin, JAN2011
Occupational Exposure Limit-THE PHILIPPINEStime-weighted average 5 ppm (22 mg/m3), Skin, JAN1993
Occupational Exposure Limit-TURKEYtime-weighted average 5 ppm (22 mg/m3), Skin, JAN1993
Occupational Exposure Limit-UNITED KINGDOMtime-weighted average 0.2 ppm (0.89 mg/m3), skin, OCT2007
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)8H time-weighted average 5 ppm (22 mg/m3) (skin)CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 5 ppm (22 mg/m3) (skin)CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 5 ppm (22 mg/m3) (skin)CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 5 ppm (22 mg/m3) (skin)CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Institute for Occupational Safety and Health (NIOSH) Recommended Exposure Level TO o-TOLUIDINE-airCarcinogen lowest feasible concentration (Sk)NIOSH* DHHS #92-100,1992
National Occupational Exposure Survey 1983Hazard Code 73470; Number of Industries 32; Total Number of Facilities 2517; Number of Occupations 25; Total Number of Employees Exposed 30019; Total Number of Female Employees Exposed 15501
National Occupational Hazard Survey 1974Hazard Code 73470; Number of Industries 21; Total Number of Facilities 2912; Number of Occupations 20; Total Number of Employees Exposed 13053

Status in Federal Agencies

OrganizationReference
EPA GENETOX PROGRAM 1988, Inconclusive: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; S cerevisiae-homozygosis
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat
EPA GENETOX PROGRAM 1988, Positive: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Positive: E coli polA without S9
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Amines, aromatic, 2002
NTP 11th Report on Carcinogens,2004:Reasonably anticipated to be a human carcinogen
OSHA ANALYTICAL METHOD #ID-73
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