Salicylic acid

RTECS #

VO0525000

CAS #

69-72-7
See: NMAM or OSHA Methods

Updated

June 2018

Molecular Weight

138.13

Molecular Formula

C₇H₆O₃

Synonyms

2-Hydroxybenzoic acid
Acido o-idrossibenzoico (Italian)
Acido salicilico (Italian)
Benzoic acid, 2-hydroxy-
Keralyt
Kyselina 2-hydroxybenzoova (Czech)
Kyselina salicylova (Czech)
o-Hydroxybenzoic acid
Orthohydroxybenzoic acid
Retarder W
SA
SAX
Verrugon

Mutation Data and Reference

System Test	Route/Organism/Tissue	Dose	Reference
DNA inhibition	oral/mouse	100 mg/kg	MUREAV 46,305,1977
DNA repair	/Bacillus subtilis	5 mg/disc	JNUDAT 34,183,1992
mutation in microorganisms	/Saccharomyes cerevisiae	1 mmol/L/3H (-enzymatic activation step)	MUREAV 60,291,1979
mutation in microorganisms	/Bacillus subtilis	5 mg/disc/48H	HPV141 -,-,2001
mutation in microorganisms	/Salmonella typhimurium	100 µg/disc (+enzymatic activation step)	JNUDAT 34,183,1992

Reproductive Effects Data and References

Route/Organism	Dose	Effect	Reference
oral/rat	40 mg/kg (20-21D pregnant)	Reproductive: Maternal effects: Parturition	PRGLBA 4,93,1973
oral/rat	1050 mg/kg (8-14D pregnant)	Reproductive: Maternal effects: Uterus, cervix, vagina Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants) Reproductive: Effects on fertility: Litter size (e.g., # fetuses per litter; measured before birth)	SEIJBO 13,73,1973
oral/rat	1050 mg/kg (8-14D pregnant)	Reproductive: Specific developmental abnormalities: Central nervous system Reproductive: Specific developmental abnormalities: Craniofacial (including nose and tongue) Reproductive: Specific developmental abnormalities: Musculoskeletal system	SEIJBO 13,73,1973
oral/rat	700 mg/kg (8-14D pregnant)	Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) Reproductive: Specific developmental abnormalities: Musculoskeletal system	SKEZAP 14,549,1973
oral/rat	350 mg/kg (8-14D pregnant)	Reproductive: Effects on embryo or fetus: Extra embryonic structures (e.g., placenta, umbilical cord)	SKEZAP 14,549,1973
oral/mouse	1 gm/kg (17D pregnant)	Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus)	APTOA6 35,107,1974
oral/mouse	500 mg/kg (9D pregnant)	Reproductive: Specific developmental abnormalities: Musculoskeletal system	APTOA6 35,107,1974
subcutaneous/rat	380 mg/kg (9D pregnant)	Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants) Reproductive: Effects on embryo or fetus: Fetotoxicity (except death, e.g., stunted fetus) Reproductive: Other developmental abnormalities	BCPCA6 22,407,1973

Acute Toxicity Data and References

Route/Organism	Dose	Effect	Reference
In Vitro/Human, leukemia cells	Inhibitor Concentration (5 percent kill): 552 mg/L/30M	In Vitro Toxicity Studies: Cell counting	TIVIEQ 27,857,2013
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): >100 µg/mL/48H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	FTRPAE 99,261,2014
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): 4331.38 µmol/L	In Vitro Toxicity Studies: Cell Viability (cell death), unspecified assay	FTRPAE 95,187,2014
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): >250 µmol/L/24H	In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.	TIVIEQ 29,582,2015
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): 581 µmol/L	In Vitro Toxicity Studies: Other assays Biochemical: Metabolism (intermediary): Effect on mitochondrial function	TIVIEQ 34,161,2016
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): 766 µmol/L/1H	In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.	TIVIEQ 34,161,2016
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): 581 µmol/L/1H	Biochemical: Metabolism (intermediary): Glycolytic	TIVIEQ 34,161,2016
In Vitro/Human, lung tumor	Inhibitor Concentration (50 percent kill): 12.01 µg/mL/48H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	FTRPAE 99,261,2014
In Vitro/Human, lymphocyte	Inhibitor Concentration (30 percent kill): >200 mg/L/45H	In Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc.	TIVIEQ 29,901,2015
In Vitro/Human, lymphocyte	Inhibitor Concentration (50 percent kill): >200 mg/L/45H	In Vitro Toxicity Studies: Other assays	TIVIEQ 29,901,2015
In Vitro/Human, monocyte	Inhibitor Concentration Low: 500 µmol/L/48H	In Vitro Toxicity Studies: Cell viability (dye exclusion): trypan blue assay etc.	TXAPA9 245,211,2010
In Vitro/Human, skin	Inhibitor Concentration (20 percent kill): 345.3 mg/L/21H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 27,314,2013
In Vitro/Human, skin	Inhibitor Concentration (50 percent kill): >2000 µmol/L/48H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 29,688,2015
In Vitro/Human, skin	Inhibitor Concentration (50 percent kill): >2000 µmol/L/48H	In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.	TIVIEQ 29,688,2015
In Vitro/Human, skin	Inhibitor Concentration (30 percent kill): >2000 µmol/L/48H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 29,688,2015
In Vitro/Human, skin	Inhibitor Concentration (30 percent kill): >2000 µmol/L/48H	In Vitro Toxicity Studies: Cell metabolic activity: Alamar Blue assay etc.	TIVIEQ 29,688,2015
In Vitro/Human, skin	Inhibitor Concentration Low: 50 µmol/L/24H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation	TXAPA9 283,147,2015
In Vitro/Human, skin	Inhibitor Concentration Low: 60 mg/well/10M	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TIVIEQ 27,1476,2013
In Vitro/mra	Inhibitor Concentration (25 percent kill): 2656.2 mg/L/24H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.	TOLED5 216,65,2013
In Vitro/mra	Inhibitor Concentration Low: 1328.1 mg/L/24H	Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation	TOLED5 216,65,2013
In Vitro/paa	Inhibitor Concentration Low: 0.5 mmol/L/20M	Biochemical: Metabolism (intermediary): Effect on mitochondrial function	TXCYAC 302,51,2012
In Vitro/Pig, spermatozoa	Inhibitor Concentration (50 percent kill): >1000 µmol/L/30M	In Vitro Toxicity Studies: Other assays	TIVIEQ 29,582,2015
In Vitro/Rat, liver	Inhibitor Concentration Low: 5 mmol/L/48H	In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc. In Vitro Toxicity Studies: Other assays	TXAPA9 249,140,2010
inhalation/mammal (species unspecified)	lethal concentration (50 percent kill): >300 mg/m³		GISAAA 57(7-8),31,1992
intraperitoneal/mouse	lethal dose (50 percent kill): 300 mg/kg	Behavioral: Somnolence (general depressed activity) Lung, Thorax, or Respiration: Respiratory depression	GNRIDX 3,675,1969
intraperitoneal/rat	lethal dose (50 percent kill): 157 mg/kg	Behavioral: Somnolence (general depressed activity) Behavioral: Tremor Nutritional and Gross Metabolic: Body temperature decrease	GISAAA 57(7-8),31,1992
intravenous/mouse	lethal dose (50 percent kill): 184 mg/kg	Behavioral: Convulsions or effect on seizure threshold	YKKZAJ 91,550,1971
oral/cat	lethal dose (50 percent kill): 400 mg/kg		HBTXAC 5,148,1959
oral/mouse	lethal dose (50 percent kill): 480 mg/kg		HBTXAC 5,148,1959
oral/rabbit	lethal dose (50 percent kill): 1300 mg/kg		NIIRDN 6,291,1982
skin/man	lowest published toxic dose: 57 mg/kg	Ear: Tinnitus	JAMAAP 244,660,1980
skin/rat	lethal dose (50 percent kill): >2 gm/kg	Liver: Other changes Skin and Appendages: Other: Hair	ATDAEI 15(Suppl 1),S81,1996
skin/woman	lowest published toxic dose: 111 mg/kg/10D- intermittent	Ear: Change in acuity Cardiac: Pulse rate increased without fall in BP Nutritional and Gross Metabolic: Body temperature increase	PGMJAO 70,146,1994
subcutaneous/mouse	lethal dose (60 percent kill): 520 mg/kg	Cardiac: Change in rate Behavioral: Muscle weakness	AIPTAK 38,9,1930
subcutaneous/rabbit	lowest published lethal dose: 6 gm/kg		HBAMAK 4,1392,1935

Reviews

Organization	Standard	Reference
TOXICOLOGY REVIEW		AIPTAK 34,173,1928
TOXICOLOGY REVIEW		TOLED5 144,383,2003
TOXICOLOGY REVIEW		JEADV* 13,14,1999
TOXICOLOGY REVIEW		TPHSDY 24,331,2003
TOXICOLOGY REVIEW		AINCM* 7,63,2006
TOXICOLOGY REVIEW		IJTOFN 22,1,2003
TOXICOLOGY REVIEW		NBREV* 11,35,1987
TOXICOLOGY REVIEW		TXAPA9 243,239,2010
TOXICOLOGY REVIEW		FCLPH* 22,335,2008
TOXICOLOGY REVIEW		FCTOD7 50,2243,2012
TOXICOLOGY REVIEW		NETEEC 52,68,2015

Standards and Regulations

Organization	Standard	Reference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION		FEREAC 54,7740,1989
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDES	Cancelled	RBREV* -,334,1998
Occupational Exposure Limit-RUSSIA	short term exposure limit 0.1 mg/m³, Skin, JUN2003

NIOSH Documentation and Surveillance

Organization	Standard	Reference
National Occupational Exposure Survey 1983	Hazard Code 67680; Number of Industries 40; Total Number of Facilities 2133; Number of Occupations 50; Total Number of Employees Exposed 51922; Total Number of Female Employees Exposed 20096
National Occupational Hazard Survey 1974	Hazard Code 67680; Number of Industries 39; Total Number of Facilities 6955; Number of Occupations 57; Total Number of Employees Exposed 61410

Status in Federal Agencies

Organization	Reference
EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test
EPA GENETOX PROGRAM 1988, Positive: S cerevisiae-reversion
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001

Page last reviewed: November 16, 2018

Content source: National Institute for Occupational Safety and Health