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Registry of Toxic Effects of Chemical Substances (RTECS)

Pyrene

RTECS #

UR2450000

CAS #

129-00-0

Updated

April 2017

Molecular Weight

202.26

Molecular Formula

C16H10

Synonyms

Benzo(def)phenanthrene
beta-Pyrene
Coal tar pitch volatiles:pyrene (OSHA)
Pyren (German)

Skin and Eye Irritation and References

Route/OrganismDoseEffectReference
skin /rabbit 500 mg/24H mild28ZPAK -,26,1972

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
Cytogenetic Analysislung/hamster10 mg/LESKHA5 (103),64,1985
DNA adduct/Escherichia coli10 µmol/LPNCCA2 5,39,1965
DNA adduct/non-mammalian species8600 nmol/LGANMAX 6,49,1968
DNA adducttestis/salmon5 µg/1H-continuousBIJOAK 110,159,1968
DNA Damageleukocyte/human12500 nmol/LMUREAV 468,93,2000
DNA Damagelymphocyte/mouse50 µmol/LMUREAV 203,155,1988
DNA Damage/Escherichia coli461.8 µg/L/3H (+enzymatic activation step)MUREAV 515,85,2002
DNA Damagefibroblast/hamster10 µmol/L/2H (-enzymatic activation step)MUREAV 650,96,2008
micronucleus testmultiple/non-mammalian species900 nmol/LMUTAEX 4,17,1989
morphological transformembryo/hamster10 mg/LCNREA8 31,1118,1971
morphological transformkidney/hamster62500 µg/LPMRSDJ 1,626,1981
morphological transformembryo/mouse10 mg/LEMMUEG 12,21,1988
morphological transformembryo/mouse10 mg/L/12DMUREAV 588,7,2005
mutation in mammalian somatic cellslymphocyte/mouse1510 µmol/LMUTAEX 3,193,1988
mutation in mammalian somatic cellsembryo/rat10 mg/LJTEHD6 4,79,1978
mutation in mammalian somatic cellsother cell types/human12 µmol/LMUREAV 130,127,1984
mutation in microorganisms/Escherichia coli1 µg/plate (+enzymatic activation step)PMRSDJ 1,387,1981
mutation in microorganismslymphocyte/mouse10100 nmol/L (+enzymatic activation step)MUTAEX 3,193,1988
mutation in microorganisms/Salmonella typhimurium5 µg/plate (+enzymatic activation step)DTESD7 2,249,1977
mutation in microorganisms/Salmonella typhimurium300 ng/plate (-enzymatic activation step)ENMUDM 6(Suppl 2),1,1984
mutation in microorganisms/Salmonella typhimurium0.5 nmol/plate/48H (-enzymatic activation step)MUREAV 557,99,2004
mutation in microorganisms/Salmonella typhimurium4 mg/L/72H (+enzymatic activation step)MUREAV 558,181,2004
mutation in microorganisms/Salmonella typhimurium0.224 mg/L/48H (+enzymatic activation step)MUREAV 750,12,2013
mutation in microorganisms/Salmonella typhimurium50 µg/plate/49H (+enzymatic activation step)MUREAV 747,36,2012
other mutation test systems/Escherichia coli50 nmol/tubeMUREAV 147,79,1985
sister chromatid exchangelymphocyte/human100 µmol/LMUREAV 211,77,1989
Unscheduled DNA Synthesisliver/rat2500 µmol/LCNREA8 42,3010,1982
Unscheduled DNA Synthesisfibroblast/human100 mg/LTXCYAC 21,151,1981
Unscheduled DNA Synthesisliver/hamster100 µmol/LENMUDM 5,1,1983

Tumorigenic Data and References

Route/OrganismDoseEffectReference
skin/mouse lowest published toxic dose: 10 gm/kg/3W- intermittentTumorigenic: Equivocal tumorigenic agent by RTECS criteria

Skin and Appendages: Tumors
BJCAAI 10,363,1956

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Human, liver tumor Inhibitor Concentration (50 percent kill): 23.0 mg/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 27,1347,2013
In Vitro/Human, skin Inhibitor Concentration (50 percent kill): 1.62 mg/L/48HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 27,1347,2013
In Vitro/mlbInhibitor Concentration Low: 10 µmol/L/24HIn Vitro Toxicity Studies: Other assaysTOLED5 208,214,2012
In Vitro/Mouse, fibroblast Inhibitor Concentration (50 percent kill): 234 mg/L/48HIn Vitro Toxicity Studies: Cell viability (lysosomal damage): neutral red assay etc.TIVIEQ 27,1347,2013
In Vitro/Rat, liver Inhibitor Concentration (50 percent kill): 45.3 mg/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.TIVIEQ 27,1347,2013
In Vitro/Rat, liver Inhibitor Concentration Low: 40 µmol/L/1HIn Vitro Toxicity Studies: Other assaysTOLED5 232,113,2015
inhalation/rat lethal concentration (50 percent kill): 170 mg/m3Eye: Conjunctiva irritation

Behavioral: Excitement

Behavioral: Muscle contraction or spasticity
GTPZAB 15(2),59,1971
inhalation/rat lethal concentration (50 percent kill): 170 mg/m3VCVGH* -,235,1990
intraperitoneal/mouse lethal dose (50 percent kill): 514 mg/kgPMRSDJ 1,682,1981
oral/mouse lethal dose (50 percent kill): 800 mg/kgEye: Conjunctiva irritation

Behavioral: Excitement

Behavioral: Muscle contraction or spasticity
GTPZAB 15(2),59,1971
oral/mouse lowest published toxic dose: 6068 µg/kgLiver: Changes in liver weight

Endocrine: Changes in thymus weight
TOXID9 78,397,2004
oral/mouse lethal dose (50 percent kill): 800 mg/kgENTOX* -,513,2005
oral/rat lethal dose (50 percent kill): 2.7 gm/kgENTOX* -,513,2005
oral/rat lethal dose (50 percent kill): 2700 mg/kgEye: Conjunctiva irritation

Behavioral: Excitement

Behavioral: Muscle contraction or spasticity
GTPZAB 15(2),59,1971
Tank with water/Zebrafish lowest published toxic concentration: 25 µmol/L/42HReproductive: Specific developmental abnormalities: Musculoskeletal system

Reproductive: Specific developmental abnormalities: Cardiovascular (circulatory) system

Reproductive: Other developmental abnormalities
TXAPA9 272,656,2013
unreported route/mouse lethal dose (50 percent kill): 800 mg/kgVCVGH* -,235,1990

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
intraperitoneal/rat lowest published toxic dose: 404 mg/kg/7D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gainARTODN 77,183,2003
multiple/rat lowest published toxic dose: 303.39 mg/kg/3D- intermittentLung, Thorax, or Respiration: Other changes

Liver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
ARTODN 81,169,2007
oral/mouse lowest published toxic dose: 21 mg/kg/7D- intermittentBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Other proteins
TOXID9 78,398,2004
oral/mouse lowest published toxic dose: 35 mg/kg/7D- intermittentBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Biochemical: Metabolism (intermediary): Lipids including transport

Biochemical: Metabolism (intermediary): Other proteins
TOXID9 78,398,2004
oral/mouse lowest published toxic dose: 42475 µg/kg/7D- intermittentLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Multiple enzyme effects
TOXID9 78,397,2004
oral/mouse lowest published toxic dose: 820 mg/kg/4D- intermittentLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other transferases
TXCYAC 238,147,2007
oral/rat lowest published toxic dose: 12150 mg/kg/45D- intermittentBehavioral: Muscle contraction or spasticity

Blood: Normocytic anemia

Blood: Changes in erythrocyte (RBC) count
GTPZAB 15(2),59,1971
unreported route/mouse lowest published toxic dose: 180 mg/kg/12D- intermittentImmunological Including Allergic: Uncharacterized (multiple organ involvement)TOXID9 48,13,1999

Reviews

OrganizationStandardReference
International Agency for Research on Cancer (IARC)Cancer Review:Animal Inadequate EvidenceIMEMDT 32,431,1983
International Agency for Research on Cancer (IARC)Cancer Review:Human No Adequate DataIMEMDT 32,431,1983
International Agency for Research on Cancer (IARC)Cancer Review:Group 3IMSUDL 7,56,1987
TOXICOLOGY REVIEWMUREAV 558,155,2004
TOXICOLOGY REVIEWMUREAV 558,7,2004
TOXICOLOGY REVIEWMUREAV 540,89,2003
TOXICOLOGY REVIEWTOLED5 140-141,353,2003
TOXICOLOGY REVIEWMUREAV 567,401,2004
TOXICOLOGY REVIEWMUREAV 589,208,2005
TOXICOLOGY REVIEWENTOX* -,513,2005
TOXICOLOGY REVIEWMUREAV 636,95,2007
TOXICOLOGY REVIEWMUREAV 636,144,2007
TOXICOLOGY REVIEWMUREAV 636,36,2007
TOXICOLOGY REVIEWMUTAEX 23,271,2008
TOXICOLOGY REVIEWMUREAV 760,1,2014
TOXICOLOGY REVIEWMUREAV 763,36,2014
TOXICOLOGY REVIEWTOLED5 205,279,2011
TOXICOLOGY REVIEWMUTAEX 31,17,2016
TOXICOLOGY REVIEWTXAPA9 266,67,2013
TOXICOLOGY REVIEWFCTOD7 86,144,2015

Standards and Regulations

OrganizationStandardReference
Occupational Exposure Limit-RUSSIAshort term exposure limit 0.03 mg/m3, Skin, JUN2003
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Construction)8H time-weighted average 0.2 mg/m3CFRGBR 29,1926.55,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Federal Contractors)8H time-weighted average 0.2 mg/m3CFRGBR 41,50-204.50,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (General Industry)8H time-weighted average 0.2 mg/m3CFRGBR 29,1910.1000,1994
Occupational Safety and Health Administration (OSHA) Permissible Exposure Limit (Shipyards)8H time-weighted average 0.2 mg/m3CFRGBR 29,1915.1000,1993

NIOSH Documentation and Surveillance

OrganizationStandardReference
National Occupational Exposure Survey 1983Hazard Code T1328; Number of Industries 3; Total Number of Facilities 560; Number of Occupations 3; Total Number of Employees Exposed 9387
National Occupational Hazard Survey 1974Hazard Code T1328; Number of Industries 1; Total Number of Facilities 11; Number of Occupations 1; Total Number of Employees Exposed 32

Status in Federal Agencies

OrganizationReference
ATSDR TOXICOLOGY PROFILE (NTIS** PB/95/264370)
EPA GENETOX PROGRAM 1988, Inconclusive: Carcinogenicity-mouse/rat; Mammalian micronucleus
EPA GENETOX PROGRAM 1988, Inconclusive: In vitro SCE-nonhuman
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-BALB/c-3T3; SHE-clonal assay
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-mouse embryo
EPA GENETOX PROGRAM 1988, Negative: Cell transform.-RLV F344 rat embryo
EPA GENETOX PROGRAM 1988, Negative: Histidine reversion-Ames test; In vivo SCE-nonhuman
EPA GENETOX PROGRAM 1988, Negative: In vitro cytogenetics-nonhuman; Host-mediated assay
EPA GENETOX PROGRAM 1988, Negative: Sperm morphology-mouse; In vitro UDS-human fibroblast
EPA GENETOX PROGRAM 1988, Negative: V79 cell culture-gene mutation
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA Section 8(d) unpublished health/safety studies
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
NIOSH Analytical Method, 1994: Polynuclear aromatic hydrocarbons by HPLC, 5506; by GC, 5515
On EPA IRIS database
OSHA ANALYTICAL METHOD #ID-58
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