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Registry of Toxic Effects of Chemical Substances (RTECS)

1H-Pyrazole-3-carbonitrile, 5-amino-1-(2,6-dichloro-4-(trifluoromethyl)phenyl)-4-((trifluoromethyl)sulfinyl)-

RTECS #

UQ4430250

CAS #

120068-37-3

Updated

June 2017

Molecular Weight

437.16

Molecular Formula

C12H4Cl2F6N4OS

Synonyms

Fipronil
M & B 46030
Regent

Mutation Data and Reference

System TestRoute/Organism/TissueDoseReference
DNA Damageother cell types/human20 µmol/L/7DMUTAEX 31,43,2016
micronucleus testother cell types/human15 µmol/L/7DMUTAEX 31,43,2016

Reproductive Effects Data and References

Route/OrganismDoseEffectReference
oral/mammal (species unspecified) 390 mg/kg (6-22D preg/10D after birth)Reproductive: Effects on newborn: BehavioralFEREAC 63,38486,1998
oral/rat 1.5 mg/kg (6-20D pregnant)Reproductive: Other effects on female

Reproductive: Specific developmental abnormalities: Central nervous system
NETEEC 45,27,2014

Acute Toxicity Data and References

Route/OrganismDoseEffectReference
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 25 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.NRTXDN 32,935,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 50 µmol/L/24HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)

In Vitro Toxicity Studies: Apoptosis in vitro
NRTXDN 32,935,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 50 µmol/L/6HIn Vitro Toxicity Studies: Apoptosis in vitroNRTXDN 32,935,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 100 µmol/L/1HBiochemical: Metabolism (intermediary): Xanthine, purine or nucleotides including urate

Biochemical: Metabolism (intermediary): Glycolytic
NRTXDN 32,935,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 100 µmol/L/6HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)TOLED5 202,133,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 100 µmol/L/6HIn Vitro Toxicity Studies: Other assaysTOLED5 202,133,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 100 µmol/L/12HIn Vitro Toxicity Studies: Other assaysTOLED5 202,133,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 25 µmol/L/24HIn Vitro Toxicity Studies: Other assaysTOLED5 202,133,2011
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 25 µmol/L/24HIn Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.

In Vitro Toxicity Studies: Apoptosis in vitro

In Vitro Toxicity Studies: Other assays
TOLED5 211,18,2012
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 25 µmol/L/24HIn Vitro Toxicity Studies: Cell membrane integrity: Cytoplasmic enzymes leakage (lactate dehydrogenase, ATP enzymes etc.)

In Vitro Toxicity Studies: Cell viability (mitochondrial reductase assays): MTT, XTT, MTS, WSTs assays etc.
TOLED5 223,25,2013
In Vitro/Human, neuroblastoma Inhibitor Concentration Low: 50 µmol/L/24HIn Vitro Toxicity Studies: Apoptosis in vitroTOLED5 223,25,2013
In Vitro/Mouse, fibroblast Inhibitor Concentration Low: 10 µmol/L/8DIn Vitro Toxicity Studies: Cell differentiation

Biochemical: Metabolism (intermediary): Lipids including transport
FCTOD7 92,217,2016
In Vitro/Mouse, neuroblastom Inhibitor Concentration Low: 1 µmol/L/24HIn Vitro Toxicity Studies: Other assaysTOLED5 201,86,2011
In Vitro/Rat, neurons Inhibitor Concentration Low: 50 µmol/L/30MIn Vitro Toxicity Studies: Cell membrane integrity (prelabeled cells): release of radioactive isotopes ([51Cr], [3H]-thymidine, [3H]-proline, [35S]- or [75Se]-methionine, 5-[125I]-2-deoxy-uridine) or fluorescent dyes (bis-carboxyethyl-carboxyfluorescein (BCECF) or calcein-AM)NRTXDN 33,1048,2012
In Vitro/Rat, neurons Inhibitor Concentration Low: 40 µmol/L/1HIn Vitro Toxicity Studies: Other assaysNRTXDN 44,204,2014
In Vitro/Rat, neurons Inhibitor Concentration (50 percent kill): 0.38 µmol/L/10MIn Vitro Toxicity Studies: Other assaysNRTXDN 40,75,2014
In Vitro/Rat, neurons Inhibitor Concentration (50 percent kill): 0.4 µmol/L/20MIn Vitro Toxicity Studies: Other assaysNETOX* 48,152,2015
In Vitro/Rat, neurons Inhibitor Concentration Low: 50 µmol/L/60MIn Vitro Toxicity Studies: Other assaysNETOX* 49,79,2015
inhalation/rat lethal concentration (50 percent kill): 0.39 gm/m3ENTOX* -,334,2005
oral/mammal (species unspecified) lethal dose (50 percent kill): 92 mg/kgFEREAC 63,38488,1998
oral/man lowest published lethal dose: 1.43 mL/kgBehavioral: Convulsions or effect on seizure threshold

Behavioral: Coma

Skin and Appendages: Other: Sweating
JTCTDW 42,955,2004
oral/mouse lethal dose (50 percent kill): 95 mg/kgENTOX* -,334,2005
oral/rat lowest published toxic dose: 100 mg/kgBrain and Coverings: Changes in surface EEG

Behavioral: Altered sleep time (including change in righting reflex)

Behavioral: Change in motor activity (specific assay)
TXCYAC 214,67,2005
oral/rat lethal dose (50 percent kill): 97 mg/kgENTOX* -,334,2005
oral/rat lowest published toxic dose: 10 mg/kgNutritional and Gross Metabolic: Body temperature decreaseTXAPA9 282,184,2015
oral/rat lowest published toxic dose: 25 mg/kgCardiac: EKG changes not diagnostic of above

Nutritional and Gross Metabolic: Body temperature decrease
TXAPA9 282,184,2015
oral/rat lowest published toxic dose: 5 mg/kgBiochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammationTXAPA9 282,161,2015
oral/wild bird lethal dose (50 percent kill): 1120 mg/kgENTOX* -,334,2005
skin/rabbit lethal dose (50 percent kill): 354 mg/kgENTOX* -,334,2005
skin/rat lethal dose (50 percent kill): >2 gm/kgENTOX* -,334,2005

Other Multiple Dose Data and References

Route/OrganismDoseEffectReference
oral/Dog lowest published toxic dose: 910 mg/kg/13W- intermittentBehavioral: Tremor

Behavioral: Convulsions or effect on seizure threshold

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
NTIS** OTS0555596
oral/mouse lowest published toxic dose: 288 mg/kg/90D- continuousNutritional and Gross Metabolic: Weight loss or decreased weight gainFEREAC 70,49599,2005
oral/mouse lowest published toxic dose: 554 mg/kg/6W- continuousBehavioral: Irritability

Liver: Changes in liver weight

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** OTS0555580
oral/rat lowest published toxic dose: 60 mg/kg/6D- intermittentBrain and Coverings: Other degenerative changes

Biochemical: Metabolism (intermediary): Other
TOXID9 78,228,2004
oral/rat lowest published toxic dose: 75 mg/kg/5D- intermittentLiver: Other changes

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)
TOXID9 90,373,2006
oral/rat lowest published toxic dose: 173.7 mg/kg/90D- continuousLiver: Changes in liver weight

Endocrine: Changes in thyroid weight

Biochemical: Metabolism (intermediary): Plasma proteins not involving coagulation
FEREAC 70,49599,2005
oral/rat lowest published toxic dose: 420 mg/kg/2W- continuousBehavioral: Food intake (animal)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** OTS0571733
oral/rat lowest published toxic dose: 336 mg/kg/4W- continuousLiver: Other changes

Liver: Changes in liver weight

Blood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)
NTIS** OTS0571374
oral/rat lowest published toxic dose: 1638 mg/kg/13W- continuousLiver: Other changes

Liver: Changes in liver weight

Endocrine: Changes in thyroid weight
NTIS** OTS0571976
oral/rat lowest published toxic dose: 10950 mg/kg/1Y- continuousKidney, Ureter, and Bladder: Other changes

Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field
NTIS** OTS0537614
oral/rat lowest published toxic dose: 43.07 mg/kg/2Y- continuousBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Endocrine: Other changes
FEREAC 70,49599,2005
oral/rat lowest published toxic dose: 56.94 mg/kg/2Y- continuousBlood: Changes in serum composition (e.g. TP, bilirubin, cholesterol)

Endocrine: Other changes
FEREAC 70,49599,2005
oral/rat lowest published toxic dose: 8322 mg/kg/2Y- continuousEndocrine: Thyroid tumors

Tumorigenic: Tumor types after systemic administration not seen spontaneously
FEREAC 70,49599,2005
oral/rat lowest published toxic dose: 42 mg/kg/14D- intermittentLiver: Other changes

Endocrine: Evidence of thyroid hypofunction

Biochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Other transferases
TXCYAC 255,38,2009
oral/rat lowest published toxic dose: 75 mg/kg/5D- intermittentBrain and Coverings: Other degenerative changes

Biochemical: Neurotransmitters or modulators (putative): Dopamine in striatum

Biochemical: Neurotransmitters or modulators (putative): Dopamine at other sites
TOXID9 -,440,2009
oral/rat lowest published toxic dose: 504 mg/kg/28D- continuousBiochemical: Metabolism (intermediary): OtherTOLED5 225,240,2014
oral/rat lowest published toxic dose: 140 mg/kg/14D- intermittentNutritional and Gross Metabolic: Weight loss or decreased weight gain

Nutritional and Gross Metabolic: Body temperature decrease
TXAPA9 282,184,2015
oral/rat lowest published toxic dose: 70 mg/kg/14D- intermittentCardiac: EKG changes not diagnostic of aboveTXAPA9 282,184,2015
oral/rat lowest published toxic dose: 70 mg/kg/14D- intermittentEndocrine: Other changes

Biochemical: Metabolism (intermediary): Effect on inflammation or mediation of inflammation
TXAPA9 282,161,2015
oral/rat lowest published toxic dose: 30 mg/kg/6D- intermittentBiochemical: Enzyme inhibition, induction, or change in blood or tissue levels: Hepatic microsomal mixed oxidase (dealkylation, hydroxylation, etc.)

Biochemical: Metabolism (intermediary): Other
FCTOD7 83,215,2015
skin/rabbit lowest published toxic dose: 210 mg/kg/21D- intermittentBehavioral: Food intake (animal)

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
FEREAC 72,46906,2007
skin/rabbit lowest published toxic dose: 280 mg/kg/28D- intermittentBehavioral: Food intake (animal)

Skin: After topical application: Primary irritation

Nutritional and Gross Metabolic: Weight loss or decreased weight gain
FEREAC 70,49599,2005

Reviews

OrganizationStandardReference
TOXICOLOGY REVIEWENTOX* -,334,2005
TOXICOLOGY REVIEWTOXIA6 49,413,2007
TOXICOLOGY REVIEWFEREAC 72,46906,2007
TOXICOLOGY REVIEWHTOPA* 1,501,2002
TOXICOLOGY REVIEWMUREAV 675,1,2009
TOXICOLOGY REVIEWFEREAC 74,15971,2009
TOXICOLOGY REVIEWNETEEC 33,608,2011
TOXICOLOGY REVIEWTXCYAC 307,35,2013
TOXICOLOGY REVIEWNETEEC 36,17,2013

Standards and Regulations

OrganizationStandardReference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDESActive registrationRBREV* -,370,1998

Status in Federal Agencies

OrganizationReference
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001
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