Glycine, N,N-bis(carboxymethyl)-, trisodium salt

RTECS #

MB8400000

CAS #

5064-31-3; 37291-81-9
See: NMAM or OSHA Methods

Updated

July 2011

Molecular Weight

257.10

Molecular Formula

C₆H₆NO₆·3Na

Synonyms

Acetic acid, nitrilotri-, trisodium salt
Cheelox nta-14, -Na3
Hampshire NTA
N,N-Bis(carboxymethyl)glycine trisodium salt
Nitrilotriacetic acid, trisodium salt
Trisodium nitrilotriacetate
Trisodium nitrilotriacetic acid

Mutation Data and Reference

System Test	Route/Organism/Tissue	Dose	Reference
Cytogenetic Analysis	lung/hamster	3 mmol/L	MUREAV 343,1,1995
micronucleus test	lung/hamster	2 mmol/L	MUREAV 343,1,1995
morphological transform	embryo/rat	495 µg/plate	JJATDK 1,190,1981
morphological transform	embryo/mouse	2 mg/L	EMMUEG 12,21,1988
other mutation test systems	lung/hamster	3 mmol/L	MUREAV 343,1,1995
specific locus test	oral/Drosophila melanogaster	5200 µmol/L	MUREAV 262,253,1991

Reproductive Effects Data and References

Route/Organism	Dose	Effect	Reference
oral/rat	39 gm/kg (8W male/8W pre-3W after birth)	Reproductive: Effects on newborn: Growth statistics (e.g., reduced weight gain)	FCTXAV 9,509,1971
oral/rat	8 gm/kg (8W male/8W pre-3W after birth)	Reproductive: Effects on fertility: Post- implantation mortality (e.g., dead and/or resorbed implants per total number of implants)	FCTXAV 9,509,1971

Tumorigenic Data and References

Route/Organism	Dose	Effect	Reference
oral/rat	lowest published toxic dose: 70300 mg/kg/2Y- continuous	Tumorigenic: Neoplastic by RTECS criteria Liver: Tumors Kidney, Ureter, and Bladder: Kidney tumors	JJIND8 66,869,1981

Acute Toxicity Data and References

Route/Organism	Dose	Effect	Reference
In Vitro/Human, liver tumor	Inhibitor Concentration (50 percent kill): 8.5 mmol/L/24H	In Vitro Toxicity Studies: Cell protein synthesis	TIVIEQ 3,189,1989
intraperitoneal/rat	lethal dose (50 percent kill): 254 mg/kg		TXAPA9 18,398,1971
oral/Dog	lethal dose (50 percent kill): >5 gm/kg	Gastrointestinal: Nausea or vomiting	TXAPA9 18,398,1971
oral/monkey	lethal dose (50 percent kill): 750 mg/kg	Gastrointestinal: Nausea or vomiting	TXAPA9 18,398,1971
oral/mouse	lethal dose (50 percent kill): 681 mg/kg		NCILB* NCI-E-C-72-3252,1973
oral/rat	lethal dose (50 percent kill): 1100 mg/kg	Gastrointestinal: Nausea or vomiting	TXAPA9 18,398,1971

Other Multiple Dose Data and References

Route/Organism	Dose	Effect	Reference
oral/rat	lowest published toxic dose: 180 gm/kg/90D- continuous	Liver: Changes in liver weight Kidney, Ureter, and Bladder: Changes in bladder weight Blood: Changes in erythrocyte (RBC) count	TXAPA9 18,398,1971
oral/rat	lowest published toxic dose: 56305 mg/kg/30D- intermittent	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis)	FCTOD7 20,433,1982
oral/rat	lowest published toxic dose: 219 gm/kg/2Y- continuous	Kidney, Ureter, and Bladder: Other changes in urine composition Nutritional and Gross Metabolic: Changes in: Other metals Related to Chronic Data: Death in the "MULTIPLE DOSE" data type field	TXAPA9 21,244,1972
oral/rat	lowest published toxic dose: 67200 mg/kg/28D- continuous	Kidney, Ureter, and Bladder: Changes in tubules (including acute renal failure, acute tubular necrosis) Kidney, Ureter, and Bladder: Other changes	NTIS** OTS0543794

Reviews

Organization	Standard	Reference
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Sufficient Evidence	IMEMDT 48,181,1990
International Agency for Research on Cancer (IARC)	Cancer Review:Animal Sufficient Evidence	IMEMDT 73,385,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Human Inadequate Evidence	IMEMDT 73,385,1999
International Agency for Research on Cancer (IARC)	Cancer Review:Group 2B	IMEMDT 73,385,1999
TOXICOLOGY REVIEW		MUREAV 584,1,2005
TOXICOLOGY REVIEW		MUREAV 654,114,2008

Standards and Regulations

Organization	Standard	Reference
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1988 PESTICIDE SUBJECT TO REGISTRATION OR RE-REGISTRATION		FEREAC 54,7740,1989
Environmental Protection Agency (EPA) Federal Insecticide, Fungicide, and Rodenticide Act (FIFRA) 1998 STATUS OF PESTICIDES	Supported	RBREV* -,298,1998

NIOSH Documentation and Surveillance

Organization	Standard	Reference
National Occupational Exposure Survey 1983	Hazard Code M0951; Number of Industries 95; Total Number of Facilities 11433; Number of Occupations 97; Total Number of Employees Exposed 224263; Total Number of Female Employees Exposed 60818
National Occupational Hazard Survey 1974	Hazard Code M0951; Number of Industries 82; Total Number of Facilities 7988; Number of Occupations 64; Total Number of Employees Exposed 77882

Status in Federal Agencies

Organization	Reference
EPA GENETOX PROGRAM 1988, Positive: Carcinogenicity-mouse/rat
EPA TSCA Section 8(b) CHEMICAL INVENTORY
EPA TSCA TEST SUBMISSION (TSCATS) DATA BASE, JANUARY 2001

Page last reviewed: November 16, 2018

Content source: National Institute for Occupational Safety and Health